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D6442

Sigma-Aldrich

JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR

Ready-to-use 2x mix for qPCR with ROX

Synonym(s):

Taq polymerase mix

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55
To inquire about this product D6442, please contact your local Merck office or dealer. Contact Technical Service

form

liquid

usage

sufficient for 20 reactions
 sufficient for 2000 reactions
 sufficient for 400 reactions

feature

dNTPs included
hotstart

concentration

2.5 units/reaction (50 μL reaction volume)

technique(s)

qPCR: suitable

color

colorless

input

purified DNA

application(s)

agriculture

compatibility

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

detection method

probe-based

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This Item
D7440S9194QR0200
JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR Ready-to-use 2x mix for qPCR with ROX

D6442

JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR

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D7440

JumpStart™ Taq ReadyMix™ for Quantitative PCR

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR SYBR® Green qPCR reagent, passive reference dye included

S9194

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR

Quantitative RT-PCR ReadyMix™ One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

QR0200

Quantitative RT-PCR ReadyMix™

usage

sufficient for 20 reactions, sufficient for 400 reactions, sufficient for 2000 reactions

usage

sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 400 reactions

usage

sufficient for 20 reactions, sufficient for 2000 reactions, sufficient for 400 reactions

usage

sufficient for 250 reactions

technique(s)

qPCR: suitable

technique(s)

qPCR: suitable

technique(s)

qPCR: suitable

technique(s)

RT-qPCR: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

input

purified DNA

input

purified DNA

input

purified DNA

input

purified RNA

General description

JumpStart™ Taq ReadyMix™ For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. This 2× concentrate master mix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.
To prepare a typical qPCR reaction, mix 25 μL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μL. Reaction volumes can be scaled down, if desired.

Application

JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR has been used:
  • For quantitative polymerase chain reaction (qPCR) of cDNA template[1][2]
  • As a component of PCR reaction mix for amplification of DNA extracted from peripheral blood leucocytes[3]
  • For qPCR amplifications using SYBR
  • For multiplex PCR
  • For reduction of primer dimers

Features and Benefits

  • JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • When performing large number of PCR reactions, JumpStart™ Taq ReadyMix™ reduces preparation time and the risk of contamination from multiple pipetting steps
  • This master mix allows consistent batch-to-batch and reaction-to-reaction performance
  • Internal Reference Dye is provided for reaction normalization

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

Quantity

Use 2.5 units per reaction.

Legal Information

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

W1754

Water, PCR Reagent

Signal Word

Danger

Hazard Statements

H272,H315,H317,H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Ox. Liq. 3 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

5.1B - Oxidizing hazardous materials

Regulatory Information

新产品

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCR4995
    SLCR5694
    SLCR3624
    SLCP9406
    SLCL5603

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    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

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    Adrien Chabot et al.
    Genetics, 176(4), 2069-2076 (2007-06-15)
    Most phenotypic differences between human and chimpanzee are likely to result from differences in gene regulation, rather than changes to protein-coding regions. To date, however, only a handful of human-chimpanzee nucleotide differences leading to changes in gene regulation have been
    Malgorzata Gozdecka et al.
    Cell reports, 9(4), 1361-1374 (2014-12-03)
    JNK and p38 phosphorylate a diverse set of substrates and, consequently, can act in a context-dependent manner to either promote or inhibit tumor growth. Elucidating the functions of specific substrates of JNK and p38 is therefore critical for our understanding
    Evrim Komurcu-Bayrak et al.
    Clinica chimica acta; international journal of clinical chemistry, 383(1-2), 110-115 (2007-06-09)
    We evaluated the relationship of the lipoprotein lipase (LPL) S447X variant with serum lipid levels and the metabolic syndrome (MS) in the Turkish Adult Risk Factor (TARF) study. This is the first study examining this LPL variant in the Turkish
    Irene B Hanning et al.
    Methods in molecular biology (Clifton, N.J.), 733, 159-170 (2011-03-25)
    Next-generation sequencing (NGS) is a powerful tool that can be utilized to profile and compare microbial populations. By amplifying a target gene present in all bacteria and subsequently sequencing amplicons, the bacteria genera present in the populations can be identified
    Bilge Ozsait et al.
    Anadolu kardiyoloji dergisi : AKD = the Anatolian journal of cardiology, 8(5), 324-330 (2008-10-14)
    In this study, our aim was to investigate the association of cholesterol ester transfer protein (CETP) TaqIB polymorphism with the likelihood of metabolic syndrome (MetS). Study was designed as a cross-sectional analysis of the Turkish Adult Risk Factor follow-up study.
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