S5193
SYBR® Green JumpStart™ Taq ReadyMix™
for quantitative PCR, without MgCl2 in buffer
Synonym(s):
Hot start PCR master mix, sybr green PCR master mix, sybr green qPCR
About This Item
Quality Level
form
liquid
usage
sufficient for 100 reactions
sufficient for 20 reactions
sufficient for 400 reactions
feature
dNTPs included
hotstart
concentration
1.25 units/reaction (50 μL reaction volume)
technique(s)
qPCR: suitable
color
colorless
input
purified DNA
suitability
suitable for PCR
application(s)
agriculture
compatibility
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000
detection method
SYBR® Green
shipped in
wet ice
storage temp.
−20°C
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General description
Application
- During RNA extraction, reverse transcription and Quantitative PCR
- During analysis of leptin and leptin receptor expression. (PCR and melting curve analyses were performed)
- For tissue RNA Isolation. (PCR and melting curve analyses were performed)
- in real-time PCR (RT-PCR) of reverse-transcribed cDNA template
- For qPCR amplifications using SYBR
- For multiplex PCR
- to prepare the real-time PCR mixture
- in the quantitative analysis of gene expression by real time, quantitative polymerase chain reaction (qPCR)
- to determine the mRNA expression of nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2) gene by qPCR
Features and Benefits
- Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required
- JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
- SYBR® Green JumpStart™ Taq ReadyMix for SYBR based qPCR is formulated with MgCl2 or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments
- This master mix allows consistency from one reaction to the next
- Designed to minimize contamination
- Reduced primer dimers
- Reduced set-up time as compared to manual or wax HotStart® methods
- Allows for room temperature set-up and contains a fluorescent dye, which is ideal for qPCR applications
Packaging
20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
500RXN is packaged as 1 X 12.5 mL
Other Notes
Principle
SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.
Legal Information
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.
Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.
Protocols
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
Related Content
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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