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D4635

Sigma-Aldrich

2′-Deoxycytidine 5′-triphosphate disodium salt

≥95%

Synonym(s):

dCTP-Na2

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100 EA
¥800.36

¥800.36

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100 EA
¥800.36

About This Item

Empirical Formula (Hill Notation):
C9H14N3Na2O13P3
CAS Number:
102783-51-7
Molecular Weight:
511.12
MDL number:
MFCD00084683
UNSPSC Code:
41106305
PubChem Substance ID:
24893889
NACRES:
NA.51

¥800.36

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Quality Level

200

Assay

≥95%

form

powder

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2

InChI

1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1

InChI key

ABWVCNMFYVEBIB-CDNBRZBRSA-L

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HPTLC plates, Silica gel 60 F₂₅₄ glass plate, plate L × W 10 cm × 20 cm

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HPTLC plates, Silica gel 60 F₂₅₄

HPTLC plates, Silica gel 60 F₂₅₄ pkg of 25 plates, plate L × W 20 cm × 20 cm, glass support

115534

HPTLC plates, Silica gel 60 F₂₅₄

HPTLC aluminum sheets, Silica gel 60 F₂₅₄ pkg of 25 sheets, sheet L × W 20 cm × 20 cm, aluminum support

105548

HPTLC aluminum sheets, Silica gel 60 F₂₅₄

HPTLC plates, Silica gel 60 F₂₅₄ pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support

105642

HPTLC plates, Silica gel 60 F₂₅₄

layer thickness

200 μm

layer thickness

200 μm

layer thickness

200 μm

layer thickness

200 μm

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator: no

feature

binder Organic Polymer, fluorescent indicator

feature

binder Organic Polymer, fluorescent indicator

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

technique(s)

thin layer chromatography (TLC): suitable

packaging

pkg of 25 plates

packaging

pkg of 25 plates

packaging

pkg of 25 sheets

packaging

pkg of 50 plates

plate L × W

10 cm × 20 cm

plate L × W

20 cm × 20 cm

plate L × W

-

plate L × W

20 cm × 10 cm

General description

2′-Deoxycytidine 5′-triphosphate (dCTP) is synthesized either by de novo pathway or from the multiple phosphorylation steps of cytidine.[1] It is deaminated to deoxyuridine monophosphate (dUMP) which is convertible to deoxythymidine triphosphate (dTTP).[1]

Application

2′-Deoxycytidine 5′-triphosphate disodium salt has been used:
  • in polymerase chain reaction mix for the amplification of Alternaria (AM) toxin DNA[2]
  • to spike oligonucleotide sample for capillary electrophoresis (CE) analysis[3]
  • as a component in adenosine 5′-monophosphate (AMP) luciferase assay[4]

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Biochem/physiol Actions

2′-Deoxycytidine 5′-triphosphate (dCTP) is used to synthesize DNA by DNA polymerases[5] or reverse transcriptases. dCTP is an allosteric regulator of deoxycytidylate (dCMP) deaminase.[6] It is used in studies of nucleotide pools, nucleotide selectivity and enzyme specificity studies, such as the preferred incorporation of dCTP by Rev1, a γ-family DNA polymerase.[7]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCN6030
    SLCF4462
    SLCF4462
    SLCN6030
    SLCC0595

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Paola Ferraro et al.
    Nucleic acids research, 38(6), e85-e85 (2009-12-17)
    Eukaryotic cells contain a delicate balance of minute amounts of the four deoxyribonucleoside triphosphates (dNTPs), sufficient only for a few minutes of DNA replication. Both a deficiency and a surplus of a single dNTP may result in increased mutation rates
    Genetics: Analysis of Genes and Genomes (2009)
    Xiaoli Xiang et al.
    Analytical biochemistry, 460, 22-28 (2014-05-27)
    A luciferase-based method was developed for measurement of 5'-adenylylsulfate (APS) reductase (APR), an enzyme of the reductive sulfate assimilation pathway in prokaryotes and plants. APR catalyzes the two-electron reduction of APS and forms sulfite and adenosine 5'-monophospahate (AMP). The luciferase-based
    Dynamics of pyrimidine deoxynucleoside triphosphate pools in relationship to DNA synthesis in 3T6 mouse fibroblasts
    Nicander B and Reichard P
    Proceedings of the National Academy of Sciences, 80(5), 1347-1351 (1983)
    Irene Lee et al.
    Biochimica et biophysica acta, 1804(5), 1064-1080 (2009-09-08)
    DNA is a remarkable macromolecule that functions primarily as the carrier of the genetic information of organisms ranging from viruses to bacteria to eukaryotes. The ability of DNA polymerases to efficiently and accurately replicate genetic material represents one of the
    View All Related Papers

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