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Safety Information

D1152

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - high glucose

HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture

Synonym(s):

DME, DMEM

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10 L
CN¥497.08
10 X 1 L
CN¥437.25
50 L
CN¥1,790.63

CN¥497.08

Please contact Customer Service for Availability
Request a Bulk Order
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10 L
CN¥497.08
10 X 1 L
CN¥437.25
50 L
CN¥1,790.63

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

CN¥497.08

Please contact Customer Service for Availability
Request a Bulk Order

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

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1 of 4

This Item
D7777D6171D1145
Sigma-Aldrich

D1152

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

D7777

Dulbecco′s Modified Eagle′s Medium - high glucose

SAFC

D6171

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

D1145

Dulbecco′s Modified Eagle′s Medium - high glucose

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

liquid

form

liquid

components

sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

components

glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes

components

sodium pyruvate: no
HEPES: 25 mM
glucose: high
NaHCO3: yes
L-glutamine: no
phenol red: yes

components

L-glutamine: no
sodium pyruvate: no
glucose: high
phenol red: no
NaHCO3: yes
HEPES: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

400

Quality Level

400

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Quantity

Formulated to contain 17.3 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000433033
    0000433033
    0000433056
    0000433057
    0000433057

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    Jiping Sun et al.
    Journal of cellular and molecular medicine, 22(10), 4840-4855 (2018-07-20)
    BMSCs are important in replacement therapy of diabetic nephropathy (DN). MiR-124a exerts effect on the differentiation capability of pancreatic progenitor cells. The objective of this study was to explore the molecular mechanisms, the functions of miR-124a and bone marrow mesenchymal
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    Protocols

    Powdered Media Preparation Instructions

    Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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