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D5523

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - low glucose

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DME, DMEM

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About This Item

MDL number:
MFCD00217342
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low

shipped in

ambient

storage temp.

2-8°C

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General description

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.

Application

Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human and murine mesenchymal stem cells (MSCs).

Quantity

Formulated to contain 10.0 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate.

also commonly purchased with this product

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Description
Pricing

supplement

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Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the formulation of Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?

    The formulation can be found on the DMEM formulation sheet at the following link.

  4. How do I prepare Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?

    Measure out 90% of final required volume of water. Water temperature should be 15-20 °C.While gently stirring the water, add the powdered medium. Stir until dissolved. Do NOT heat.Rinse original package with a small amount of water to remove all traces of powder. Add to solution in step 2.To the solution in step 3, add 3.7 g sodium bicarbonate or 49.3 ml of sodium bicarbonate solution [7.5%w/v] for each liter of final volume of medium being prepared. Stir until dissolved.While stirring, adjust the pH of the medium to 0.1-0.3 pH units below the desired pH since it may rise during filtration. The use of 1N HCl or 1N NaOH is recommended.Add additional water to bring the solution to final volume.Sterilize immediately by filtration using a membrane with a porosity of 0.22 microns.Aseptically dispense medium into sterile container.

  5. How do I store Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose, dry powder?  How do I store the reconstituted medium?

    Store the dry powdered medium at 2-8 °C under dry conditions and liquid medium at 2-8 °C in the dark.  The nature of supplements added may affect storage conditions and shelf life of the medium. The product label bears the lot-specific expiration date.

  6. What sodium bicarbonate should I use with Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?

    Sodium Bicarbonate powder (Product No. S5761) or Sodium Bicarbonate Solution, 7.5% (Product No. S8761) is recommended.  For more information, consult the product datasheet.

  7. Do you offer a liquid version of Dulbecco’s Modified Eagle’s Medium - low glucose?

    Product No. D6046 is the fully supplemented liquid form of the original Dulbecco's formulation, offered as Product No. D5523, with one key exception.  Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.  D5546 is the same but does not contain L-glutamine.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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