Skip to Content
Merck
CN
All Photos(1)

Documents

Safety Information

D5796

Sigma-Aldrich

DMEM - high glucose

With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, sterile liquid, suitable for cell culture

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

also commonly purchased with this product

Product No.
Description
Pricing

D8537

Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

D1145

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture

R8758

RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

F1051

Fetal Bovine Serum, Canada origin, sterile-filtered, suitable for cell culture

CLS430769

Corning® bottle-top vacuum filter system, cellulose acetate membrane, pore size 0.22 μm, membrane area 33.2 cm2, filter capacity 500 mL

recommended

Product No.
Description
Pricing

related product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Christian Kirschneck et al.
Scientific reports, 7(1), 14751-14751 (2017-11-09)
Meaningful, reliable and valid mRNA expression analyses by real-time quantitative PCR (RT-qPCR) can only be achieved, if suitable reference genes are chosen for normalization and if appropriate RT-qPCR quality standards are met. Human periodontal ligament (hPDL) fibroblasts play a major
Refael Minnes et al.
Scientific reports, 7(1), 4381-4381 (2017-07-01)
The vast majority of cancer related deaths are caused by metastatic tumors. Therefore, identifying the metastatic potential of cancer cells is of great importance both for prognosis and for determining the correct treatment. Infrared (IR) spectroscopy of biological cells is
Saabah B Mahbub et al.
Scientific reports, 7(1), 15792-15792 (2017-11-19)
Bioimaging using endogenous cell fluorescence, without any external biomarkers makes it possible to explore cells and tissues in their original native state, also in vivo. In order to be informative, this label-free method requires careful multispectral or hyperspectral recording of
Tomoyuki Muroi et al.
Reproduction (Cambridge, England), 154(6), 755-764 (2017-09-16)
G-protein-coupled receptors (GPCRs) participate in diverse physiological functions and are promising targets for drug discovery. However, there are still over 140 orphan GPCRs whose functions remain to be elucidated.
Kai-Liang Zhang et al.
OncoTargets and therapy, 12, 6515-6523 (2019-10-17)
The efficacy of traditional therapies for oral carcinoma (OC) is limited. Oncolytic adenovirus, a novel strategy of cancer therapy, shows potential use in OC treatment. However, its clinical application is limited by pre-existing neutralizing antibodies. Thus, this study aimed to
View All Related Papers

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Protocols

Primary Human Hepatic Sinusoidal Endothelial Cells Culture Protocol

Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Related Content

Primary Human Hepatic Stellate Cells Culture Protocol

Discover our collection of primary human hepatic stellate cells and protocol for thawing, plating, and growing stellate cells. Find our stellate culture maintenance media formulation.

Cell Culture Media Preparation Using MilliShot™ Single Dose Antibiotics

Learn how to use our cell culture tested, ready-to-use MilliShot™ single dose antibiotic solutions, conveniently packaged in one-time use vials.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service