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S5761

Sigma-Aldrich

Sodium bicarbonate

powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Synonym(s):

Sodium hydrogen carbonate, soda bicarbonate

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About This Item

Linear Formula:
NaHCO3
CAS Number:
144-55-8
Molecular Weight:
84.01
Beilstein:
4153970
EC Number:
205-633-8
MDL number:
MFCD00003528
UNSPSC Code:
12161702
PubChem Substance ID:
24899673
NACRES:
NA.75

grade

for molecular biology

Quality Level

300

product line

BioReagent

Assay

99.5-100.5%

form

powder

quality

300

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

pH

6.8-7.4

pKa (25 °C)

(1) 6.37, (2) 10.25 (carbonic acid)

solubility

water: soluble 100 mg/mL

density

2.16 g/mL at 25 °C (lit.)

SMILES string

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

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General description

Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.

Application

Sodium bicarbonate has been used:
  • as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)
  • as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells
  • as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells
  • as a component of medium used for cell culture of human embryonic kidney cells
  • as a component of Beltsville thawing solution used for the dilution of semen

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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L Keskintepe et al.
Biology of reproduction, 55(2), 333-339 (1996-08-01)
The objective was to establish an in vitro system in which bovine oocytes can be matured, fertilized, and cultured up to the blastocyst stage without support of serum, BSA, or somatic cells. Media consisted of modified tissue culture medium 199
E Meyer et al.
The European journal of neuroscience, 11(3), 1105-1108 (1999-04-02)
The human slow poke (hSlo) K+ channel was tagged with GFP (green fluorescent protein) at the N-terminus of its alpha-subunit. The fusion protein was expressed transiently in HEK293 cells; it formed functional voltage-gated channels as shown by whole cell patch-clamp
K Okuda et al.
Biology of reproduction, 61(4), 1017-1022 (1999-09-24)
There is sufficient evidence to prove that tumor necrosis factor alpha (TNFalpha) modulates bovine corpus luteum (CL) function. Our previous study demonstrated that functional TNFalpha receptors are present on luteal cells in bovine CL throughout the estrous cycle. The purpose
M Solís et al.
Research in veterinary science, 83(3), 403-409 (2007-03-21)
Porcine rubulavirus (PoRV), also known as blue eye disease (BED) of swine, causes respiratory and reproductive problems in pigs at several developmental stages. To study the effect of PoRV infection on semen production, five boars were infected with 1 x
Chang-Hwan Park et al.
Methods in molecular biology (Clifton, N.J.), 407, 311-322 (2008-05-06)
In this chapter, we introduce a co-culture protocol for human embryonic stem (hES) cell differentiation in which dopamine (DA) neurons with midbrain-specific markers are efficiently derived. Human ES cells on a feeder layer of stromal cells are induced to differentiate
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