Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
All Photos(3)

Key Documents

Safety Information

CMC0014

Sigma-Aldrich

BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

Synonym(s):

Competent E. coli cells

Sign Into View Organizational & Contract Pricing

Select a Size

500 G
CN¥650.82
1 KG
CN¥1,183.73
5 KG
CN¥3,903.78

CN¥650.82

Available to ship onApril 14, 2025Details

All Photos(3)

Select a Size

Change View
500 G
CN¥650.82
1 KG
CN¥1,183.73
5 KG
CN¥3,903.78

About This Item

UNSPSC Code:
41106202
NACRES:
NA.85

CN¥650.82

Available to ship onApril 14, 2025Details

Product Name

BL21(DE3) Chemically Competent Cells, for protein expression

biological source

Escherichia coli

grade

Molecular Biology

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

shipped in

dry ice

storage temp.

−70°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
S8761PHR359131437M
Sodium bicarbonate powder, BioReagent, suitable for cell culture, suitable for insect cell culture

S5761

Sodium bicarbonate

Sodium bicarbonate solution solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

S8761

Sodium bicarbonate solution

Sodium bicarbonate certified reference material, pharmaceutical secondary standard

PHR3591

Sodium bicarbonate

Sodium bicarbonate puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.7%

31437M

Sodium bicarbonate

assay

99.5-100.5%

assay

-

assay

-

assay

≥99.7%

solubility

water: soluble 100 mg/mL

solubility

-

solubility

-

solubility

H2O: 0.5 g/10 mL, clear, colorless, water: soluble 50 g/L

Quality Level

300

Quality Level

400

Quality Level

300

Quality Level

-

grade

for molecular biology

grade

-

grade

certified reference material, pharmaceutical secondary standard

grade

ACS reagent, puriss. p.a.

pH

6.8-7.4

pH

7.8-8.2

pH

-

pH

≤8.6 (20 °C, 5%)

General description

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

The E. coli strain BL21 (DE3) has been used:

  • to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.[1]
  • BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.[2]
  • to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.[3][1]

Features and Benefits

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Components

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

related product

Product No.
Description
Pricing

L3022

LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L2897

LB Broth with agar (Lennox), Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

B11001

EnPresso® B, Growth system for expressing protein in bacteria

H9950

Hanahan′s Broth (SOB Medium), Liquid microbial growth medium

CMC0015

BL21(DE3) pLysE Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

CMC0023

CONTROLLER SIG10 Chemically Competent Cells, for controlled protein expression

CMC0024

CONTROLLER BL21(DE3) Chemically Competent Cells, for controlled protein expression

CMR0001

Recovery Medium for Expression, for use with competent cells

Storage Class Code

10 - Combustible liquids

Regulatory Information

高风险级别生物产品--病毒，疫苗等
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000346689
    SLCM7271
    SLCM4785
    SLCP0759
    SLCN4860

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Romana Koszagova et al.
    Microbial cell factories, 17(1), 139-139 (2018-09-05)
    Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction
    A validated antibody panel for the characterization of tau post-translational modifications.
    Ebru E, et al.
    Mol. Neurodegener., 12(1), 87-87 (2017)
    David B Sauer et al.
    eLife, 9 (2020-09-02)
    Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters
    Yasaman Damestani et al.
    Lasers in surgery and medicine, 48(8), 782-789 (2016-10-19)
    The development and feasibility of a novel nanocrystalline yttria-stabilized-zirconia (nc-YSZ) cranial implant has been recently established. The purpose of what we now call "window to the brain (WttB)" implant (or platform), is to improve patient care by providing a technique
    Ibrahim Torktaz et al.
    Applied microbiology and biotechnology, 102(19), 8389-8402 (2018-08-04)
    The celH gene from Clostridium thermocellum encodes a protein containing 900 residues and three components, including Cel5E, Lic26a, and carbohydrate-binding domains. Cel5E is a member of the glycoside hydrolase-5 family and is a bifunctional xylanase/cellulase enzyme. We targeted a semi-hydrophobic
    View All Related Papers

    Related Content

    BL21 Chemically Competent Cells Overview

    BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service