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Key Documents

H9950

Sigma-Aldrich

Hanahan′s Broth (SOB Medium)

Liquid microbial growth medium

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About This Item

UNSPSC Code:
12352200

grade

Molecular Biology

sterility

sterile; 0.2 μm filtered

form

liquid

suitability

suitable for nonselective for Escherichia coli and Coliforms

storage temp.

room temp

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Hanahan′s Broth (SOB Medium) Liquid microbial growth medium

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form

liquid

form

powder

form

liquid

form

powder

sterility

sterile; 0.2 μm filtered

sterility

non-sterile

sterility

sterile; 0.2 μm filtered

sterility

non-sterile

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

General description

Hanahan′s (SOB) media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

Hanahan′s (SOB) media provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4

Physical form

20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLBN5412V
    SLBL9936V

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