Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
All Photos(4)

Key Documents

Safety Information

CMC0016

Sigma-Aldrich

BL21(DE3) Electrocompetent Cells

Escherichia coli, rod shaped

Synonym(s):

BL21 strain

Sign Into View Organizational & Contract Pricing

Select a Size

1 EA
CN¥6,148.65

CN¥6,148.65

Available to ship onApril 27, 2025Details

All Photos(4)

Select a Size

Change View
1 EA
CN¥6,148.65

About This Item

UNSPSC Code:
41106202
NACRES:
NA.85

CN¥6,148.65

Available to ship onApril 27, 2025Details

Product Name

BL21(DE3) Electrocompetent Cells, for protein expression

biological source

Escherichia coli

grade

Molecular Biology

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

shipped in

dry ice

storage temp.

−70°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
581322U581321U581301U
Ascentis® C18 HPLC Column 5 μm particle size, L × I.D. 25 cm × 4.6 mm

581325U

Ascentis® C18 HPLC Column

Ascentis® C18 HPLC Column 3 μm particle size, L × I.D. 15 cm × 4.6 mm

581322U

Ascentis® C18 HPLC Column

Ascentis® C18 HPLC Column 3 μm particle size, L × I.D. 10 cm × 4.6 mm

581321U

Ascentis® C18 HPLC Column

Ascentis® C18 HPLC Column 3 μm particle size, L × I.D. 10 cm × 2.1 mm

581301U

Ascentis® C18 HPLC Column

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

pore size

100 Å

pore size

100 Å

pore size

100 Å

pore size

100 Å

General description

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Features and Benefits

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Components

  • BL21(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Storage Class Code

10 - Combustible liquids

Regulatory Information

新产品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCM4783
    SLCP1550
    SLCM4784
    SLCJ4196
    SLCJ5997

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Felix Nicolaus et al.
    eLife, 10 (2021-02-09)
    We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected
    Julianne M Troiano et al.
    eLife, 10 (2021-01-16)
    Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
    Zev A Ripstein et al.
    eLife, 9 (2020-01-10)
    The ClpXP degradation machine consists of a hexameric AAA+ unfoldase (ClpX) and a pair of heptameric serine protease rings (ClpP) that unfold, translocate, and subsequently degrade client proteins. ClpXP is an important target for drug development against infectious diseases. Although
    Salvatore Di Girolamo et al.
    Microbial cell factories, 19(1), 170-170 (2020-08-29)
    Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows
    Brandon R Lowe et al.
    eLife, 10 (2021-02-02)
    Sequencing of cancer genomes has identified recurrent somatic mutations in histones, termed oncohistones, which are frequently poorly understood. Previously we showed that fission yeast expressing only the H3.3G34R mutant identified in aggressive pediatric glioma had reduced H3K36 trimethylation and acetylation
    View All Related Papers

    Protocols

    BL21(DE3) Electrocompetent Cells

    Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service