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A9758

Sigma-Aldrich

ACES

≥99.0% (titration)

Synonym(s):

2-[(2-Amino-2-oxoethyl)azaniumyl]ethanesulfonate, 2-[(Carbamoylmethyl)amino]ethane-1-sulfonic acid, N-(2-Acetamido)-2-aminoethanesulfonic acid, N-(Carbamoylmethyl)-2-aminoethanesulfonic acid, N-(Carbamoylmethyl)taurine

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2.5 G
CN¥753.33
5 G
CN¥882.81
25 G
CN¥3,158.37
100 G
CN¥10,143.02

CN¥753.33

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2.5 G
CN¥753.33
5 G
CN¥882.81
25 G
CN¥3,158.37
100 G
CN¥10,143.02

About This Item

Linear Formula:
H2NCOCH2NHCH2CH2SO3H
CAS Number:
7365-82-4
Molecular Weight:
182.20
Beilstein:
2253770
EC Number:
230-908-4
MDL number:
MFCD00008030
UNSPSC Code:
12161700
PubChem Substance ID:
24891495
NACRES:
NA.25

CN¥753.33

Please contact Customer Service for Availability
Request a Bulk Order

Quality Level

200

Assay

≥99.0% (titration)

form

crystalline powder

pH

3.0-4.5

useful pH range

6.1-7.5

pKa (25 °C)

6.8

mp

>220 °C (dec.) (lit.)

solubility

water: 0.523 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

storage temp.

room temp

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Sodium glycocholate hydrate ≥95% (TLC)

G7132

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Sodium taurodeoxycholate hydrate BioXtra, ≥97% (TLC)

T0557

Sodium taurodeoxycholate hydrate

Sodium cholate hydrate BioXtra, ≥99%

C6445

Sodium cholate hydrate

Sodium deoxycholate monohydrate BioUltra, ≥99.0% (NT)

30968

Sodium deoxycholate monohydrate

aggregation number

1.9

aggregation number

6

aggregation number

2-3

aggregation number

3-12

description

anionic

description

anionic

description

anionic

description

anionic

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

mp

210-215 °C (subl.) (lit.)

mp

-

mp

-

mp

-

mol wt

micellar avg mol wt 900(lit.)

mol wt

micellar avg mol wt 3100

mol wt

micellar avg mol wt 900-1300

mol wt

average mol wt 1200-5000

General description

N-(2-Acetamido)-2-aminoethanesulfonic acid (ACES) is a zwitterionic buffer used in biochemistry and molecular biology research. It is one of the Good′s buffers with a pH range of 6.15 - 8.35. The preferred range of ACES buffer in an aqueous solution is 6.1 - 7.5.

Application

ACES has been used:

  • as a component of buffered charcoal yeast extract (BYCE) agar for plating bacteria culture and Buffered Yeast Extract (BYE) liquid medium to culture bacteria[1]
  • to study the impact of the pH shift on the thermal inactivation of bacterial (Bacillus subtills) spores[2]
  • as a buffer system to study the depolymerization of starch by α-amylase during exposure to hydrostatic pressure.[3]
It is also suitable for use: as buffers for both agarose and polyacrylamide gel electrophoresis; in the isoelectric focusing of proteins; in the analysis of bacterial autolysins in a discontinuous sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) system.

Other Notes

The product is intended for laboratory use only, and it is not suitable for drug, household, or other purposes.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000418468
    0000418467
    0000418467
    0000418468
    0000423893

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    Shift of pH-value during thermal treatments in buffer solutions and selected foods
    Reineke K, et al.
    International journal of food properties (2011)
    Catalytic activity of $\beta$-amylase from barley in different pressure/temperature domains
    Heinz V, et al.
    Biotechnology Progress (2005)
    High-content assay to measure mitochondrial function and bacterial vacuole size in infected human primary macrophages
    Drame M, et al.
    STAR protocols (2023)
    P H Edelstein et al.
    Journal of clinical microbiology, 31(12), 3329-3330 (1993-12-01)
    Growth of Legionella spp. on buffered charcoal yeast extract medium supplemented with alpha-ketoglutarate and formulated with 3-(n-morpholino)propanesulfonic acid (MOPS), 3-(n-morpholino)-2-hydroxypropanesulfonic acid (MOPSO), or n-(2-acetamido)-2-aminoethanesulfonic acid (ACES) buffer was similar. With three exceptions, growth was no different in buffered yeast extract
    Christopher Oldfield et al.
    Faraday discussions, 129, 247-263 (2005-02-18)
    Water-in-oil microemulsions are thermodynamically stable single-phase dispersions of water and surfactant within a continuous oil phase. The classical ternary system, based on the surfactant sodium bis(2-ethylhexyl)sulfosuccinate ('AOT'), water and an alkane such as n-heptane, is an optically transparent monodispersion of
    View All Related Papers

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