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Merck
CN

H9882

4-Hydroxybenzhydrazide

powder, ≥97%

Synonym(s):

4-Hydroxybenzoic hydrazide, 4-Hydroxybenzoylhydrazine, p-Hydroxybenzoic acid hydrazide

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About This Item

Linear Formula:
HOC6H4CONHNH2
CAS Number:
5351-23-5
Molecular Weight:
152.15
Beilstein:
473052
EC Number:
226-326-5
MDL number:
MFCD00007605
UNSPSC Code:
12352204
PubChem Substance ID:
24278478
NACRES:
NA.32

Key Documents

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Product Name

4-Hydroxybenzhydrazide, ≥97%

biological source

synthetic

Quality Level

100

Assay

≥97%

form

powder

mp

264-266 °C (dec.) (lit.)

solubility

acetic acid: water (1:1): 25 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 360 nm; λem 425 nm (reacted with 5-hydroxymethyl-furaldehydezinc acetate)

SMILES string

NNC(=O)c1ccc(O)cc1

InChI

1S/C7H8N2O2/c8-9-7(11)5-1-3-6(10)4-2-5/h1-4,10H,8H2,(H,9,11)

InChI key

ZMZGIVVRBMFZSG-UHFFFAOYSA-N

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Application

4-Hydroxybenzhydrazide has been used:
  • for determining the concentration of maltose from starch digestion
  • for analysis of sugars from fructan containing food hydrolysate
  • in colorimetric assay for reducing sugar estimation from processed feedstuff

Biochem/physiol Actions

4-Hydroxybenzhydrazide (PAHBAH), is used for assaying α-amylase and glucoamylase activities. The assay based on PAHBAH measures non-specifically the free reducing sugar groups present in samples at 415nm colorimetrically.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000516863
0000516863
0000488260
0000488260
0000476442

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Glucoamylase (exo-1, 4-alpha-d-glucan glucanohydrolase, EC 3.2. 1.3) is the major starch-degrading enzyme secreted by the phytopathogenic fungus Colletotrichum gloeosporioides
Krause DR, et al
Microbiology, 137(10), 2463-2468 (1991)
Dongling Qiao et al.
Carbohydrate polymers, 144, 271-281 (2016-04-17)
Combined analytical techniques were used to explore the effects of alkali treatment on the multi-scale structure and digestion behavior of starches with different amylose/amylopectin ratios. Alkali treatment disrupted the amorphous matrix, and partial lamellae and crystallites, which weakened starch molecular
Efficacy of reducing sugar and phenol-sulfuric acid assays for analysis of soluble carbohydrates in feedstuffs
Hall, Mary Beth
Anim. Feed Sci. Technol., 185(1-2), 94-100 (2013)
Fang Xie et al.
International journal of biological macromolecules, 126, 525-530 (2018-12-28)
Starch-containing food normally has complex components and structure, which control its digestion kinetic properties and nutritional value. However, interactions among multiple food components and their effects on starch digestion kinetics are rarely reported. Here, we evaluated the influence of tea
Extrusion induced low-order starch matrices: Enzymic hydrolysis and structure
Zhang B, et al.
Carbohydrate Polymers, 134(10), 485-496 (2015)
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Protocols

Enzymatic Assay of Invertase

Enzymatic Assay of Invertase

转化酶的酶学测定

在测定转化酶活性时，使用了一种在410nm处的分光光度终点反应法进行测定。在55 ºC、pH 4.5条件下，一单位的转化酶每分钟可将1.0 μmole的蔗糖水解为转化糖。

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