A2576
Agarose
Ultra-low Gelling Temperature, molecular biology grade
Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan
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About This Item
Recommended Products
grade
Molecular Biology
Quality Level
type
Type IX-A
form
powder
technique(s)
electrophoresis: suitable
impurities
≤7% water
ash
≤0.4%
EEO
≤0.12
mp
≤62 °C
transition temp
gel point ≤20 °C (1.5% gel)
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This Item | A6877 | A4718 | A9539 |
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| gel strength ≥300 g/cm2 (1.5% gel) | gel strength ≥1000 g/cm2 (1.0% gel) | gel strength - | gel strength ≥1200 g/cm2 (1% gel) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| grade Molecular Biology | grade for molecular biology | grade for molecular biology | grade for molecular biology |
| transition temp gel point ≤20 °C (1.5% gel) | transition temp congealing temperature 34.5-37.5 °C (1.5% gel) | transition temp gel point ≤35 °C (3% gel) | transition temp gel point 36 °C ±1.5 °C (1.5% gel) |
General description
Application
- in thaumatin and elastase protein crystallization[4]
- in microcasting using a polydimethylsiloxane (PDMS) mold with micro-channel[5]
- in agarose micro-confinement array[6]
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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