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Merck
CN

A9883

ADA

≥98% (titration)

Synonym(s):

N-(2-Acetamido)iminodiacetic acid, N-(Carbamoylmethyl)iminodiacetic acid

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About This Item

Linear Formula:
H2NCOCH2N(CH2CO2H)2
CAS Number:
26239-55-4
Molecular Weight:
190.15
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24891404
EC Number:
247-530-0
Beilstein/REAXYS Number:
1787181
MDL number:
MFCD00008031

Key Documents

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Product Name

ADA, ≥98% (titration)

InChI key

QZTKDVCDBIDYMD-UHFFFAOYSA-N

InChI

1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13)

SMILES string

NC(=O)CN(CC(O)=O)CC(O)=O

assay

≥98% (titration)

form

powder

pH

1.5-3.0

useful pH range

6.0-7.2

pKa (25 °C)

6.6

mp

219 °C (dec.) (lit.)

solubility

1 M NaOH: 160 mg/mL, clear, colorless

application(s)

diagnostic assay manufacturing

storage temp.

room temp

Quality Level

400

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Application

Biological buffer component with sodium hydroxide and sodium chloride (pH 5.67-7.57, useful pH range 6.4-7.4). Used to prepare immobilized pH gradients.

General description

ADA is a zwitterionic buffer used in biochemistry and molecular biology. It is one of the Good buffers developed in the 1960′s to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of ADA and its physical properties. The useful range of ADA buffer in aqueous solution is 6.0 - 7.2.

Preparation Note

This product is soluble in 1 M NaOH (160mg/mL), yielding a clear, colorless solution.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000497683
0000497681
0000497681
0000497683
0000467032

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Hydrogen ion buffers for biological research
Good NE, et al.
Biochemistry, 5( (2), 467-477 (1966)
Harold Bays et al.
The Journal of clinical endocrinology and metabolism, 100(8), 3140-3148 (2015-06-02)
Despite current standard of care, many patients at high risk of cardiovascular disease (CVD) still have elevated low-density lipoprotein cholesterol (LDL-C) levels. Alirocumab is a fully human monoclonal antibody inhibitor of proprotein convertase subtilisin/kexin type 9. The objective of the
Johannan F Brandse et al.
Journal of Crohn's & colitis, 9(11), 973-981 (2015-06-28)
High serum concentrations of infliximab [IFX] and adalimumab [ADA] may be associated with adverse effects in patients with inflammatory bowel disease [IBD]. We aimed to investigate whether high anti-tumour necrosis factor [TNF] trough levels [TLs] were associated with toxicity and
Gulen Yesilbag Tonga et al.
Nature chemistry, 7(7), 597-603 (2015-06-24)
Bioorthogonal catalysis broadens the functional possibilities of intracellular chemistry. Effective delivery and regulation of synthetic catalytic systems in cells are challenging due to the complex intracellular environment and catalyst instability. Here, we report the fabrication of protein-sized bioorthogonal nanozymes through
Christopher J Smiley et al.
Journal of the American Dental Association (1939), 146(7), 508-524 (2015-06-27)
Conduct a systematic review and meta-analysis on nonsurgical treatment of patients with chronic periodontitis by means of scaling and root planing (SRP) with or without adjuncts. A panel of experts convened by the American Dental Association Council on Scientific Affairs
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Product Information Sheet - A9883

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