KCQS01
KiCqStart® SYBR® Green qPCR ReadyMix™
Low ROX™, for ABI and Stratagene instruments
Recommended Products
form
liquid
usage
sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions
feature
dNTPs included
hotstart
storage condition
protect from light
technique(s)
qPCR: suitable
color
colorless
input
purified DNA
compatibility
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI ViiA 7
for use with Agilent AriaMx
for use with Douglas Scientific IntelliQube
for use with Qiagen Rotor-Gene Q
for use with QuantStudio™
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000
detection method
SYBR® Green
shipped in
dry ice
storage temp.
−20°C
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General description
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.
Application
- for the amplification and quantification of DNA in real-time PCR (qPCR) assay
- in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR)
- in the amplification of complementary DNA (cDNA) by real-time PCR (qPCR) assay
- Gene expression
- DNA quantification
- CHiP
Features and Benefits
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Components
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
Quality
Other Notes
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Legal Information
recommended
related product
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
Protocols
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.
Related Content
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
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