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Key Documents

Safety Information

CMC0008

Sigma-Aldrich

STEADY Chemically Competent Cells

Escherichia coli, rod shaped

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1 EA
CN¥10,654.94

CN¥10,654.94

Available to ship onApril 14, 2025Details

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1 EA
CN¥10,654.94

About This Item

UNSPSC Code:
12352200

CN¥10,654.94

Available to ship onApril 14, 2025Details

Product Name

STEADY Chemically Competent Cells, for plasmid production using unstable DNA

biological source

Escherichia coli

grade

Molecular Biology

form

buffered aqueous solution

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: chemically competent
transformation efficiency: >1 x 108 cfu/μg

shipped in

dry ice

storage temp.

−70°C

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582711U53827U50630U
Ascentis® Express C18, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

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Ascentis® Express C18, 2.7 μm HPLC Column

Ascentis® Express C18, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 5 cm × 1 mm

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Ascentis® Express C18, 2.7 μm HPLC Column

Ascentis® Express C18, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 10 cm × 4.6 mm

53827U

Ascentis® Express C18, 2.7 μm HPLC Column

Ascentis® Express C18, 2.7 μm HPLC Column particle size, std dev 2.7 μm, L × I.D. 10 cm × 1.5 mm

50630U

Ascentis® Express C18, 2.7 μm HPLC Column

particle size

2.7 μm

particle size

2.7 μm

particle size

2.7 μm

particle size

std dev 2.7 μm

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

-

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

technique(s)

HPLC: suitable, UHPLC-MS: suitable, LC/MS: suitable, UHPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

-

General description

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

Features and Benefits

  • Clone unstable sequences with reliable results
  • Available as chemically competent or electrocompetent cells
  • Your choice of two packaging formats
  • High efficiency: over 1 × 108 cfu/μg (chemi) or 1 × 1010 cfu/μg (electro)
  • Excellent value

Components

  • STEADY chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning


Storage Class Code

10 - Combustible liquids

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBS4873
SLBR8265V
SLBQ7965V

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