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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
12CA5, monoclonal
Assay
>90% (HPLC and SDS-PAGE)
form
frozen liquid (11666606001)
lyophilized (11583816001)
packaging
pkg of 200 μg (11583816001)
pkg of 5 mg (11666606001 [1 ml])
manufacturer/tradename
Roche
concentration
5 mg/ml (11666606001)
isotype
IgG2bκ
epitope sequence
YPYDVPDYA
storage temp.
−20°C
Related Categories
General description
Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. The HA gene is mapped to segment 4 of influenza B viral genome.
Specificity
Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein.
Application
Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
- Dot blots
- Immunochemistry
- Immunoprecipitation
- Western blotting
Biochem/physiol Actions
Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.
Quality
Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- Dot blot: 0.1 to 1 μg/ml
- Immunfluorescence: 1-10 μg/ml
- Western blot: 0.1 to 1 μg/ml
Reconstitution
11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
13 - Non Combustible Solids
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin.
Skehel J J and Wiley D C
Annual Review of Biochemistry, 69(1), 531-569 (2000)
On the origin of the human influenza virus subtypes H2N2 and H3N2
Scholtissek C, et al.
Virology, 87(1), 13-20 (1978)
Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus.
Stevens J, et al.
Science, 312(5772), 404-410 (2006)
Jen-Liang Su et al.
Cancer research, 71(21), 6878-6887 (2011-09-14)
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Yiwei He et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(40), e2212134119-e2212134119 (2022-09-27)
Eukaryotic chromosomes are organized into structural and functional domains with characteristic replication timings, which are thought to contribute to epigenetic programming and genome stability. Differential replication timing results from epigenetic mechanisms that positively and negatively regulate the competition for limiting
Articles
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