17783
Calcein-AM
BioReagent, suitable for fluorescence, ≥90% (HPLC)
Synonym(s):
Calcein acetoxymethyl ester, Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester
Select a Size
Select a Size
About This Item
Recommended Products
product line
BioReagent
Quality Level
Assay
≥90% (HPLC)
fluorescence
λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
InChI
1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
InChI key
XKFSBWQWNMZWFA-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
Related Categories
1 of 4
This Item | NIST1549A | NIST8403 | NIST3530 |
|---|---|---|---|
| application(s) food and beverages | application(s) - | application(s) food and beverages | application(s) food and beverages |
| format matrix material | format matrix material | format matrix material | format matrix material |
| grade certified reference material | grade certified reference material | grade certified reference material | grade certified reference material |
| packaging pkg of 5 pouches x 10 g ea | packaging pkg of 5 pouches x 10 g ea | packaging pkg of 10 g | packaging pkg of 200 g |
| manufacturer/tradename NIST® | manufacturer/tradename NIST® | manufacturer/tradename NIST® | manufacturer/tradename NIST® |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level - |
General description
Application
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the solubility of Calcein-AM, Product 17783?
Sigma tests the solubility of Product No. 17783 at a concentration of 10 mg/mL in dimethyl sulfoxide (DMSO).
What is the appearance of Calcein-AM, Product No. 17783?
Product No. 17783 can be a film, oil, paste or solid.
What are the spectral properties of Calcein-AM, Product 17783?
The emission maximum for Calcein-AM is 516 nm, with excitation at 496 nm in 0.1M Tris pH 8.0 (following hydrolysis by esterase enzyme, bound to Ca(2+)).
How is Calcein-AM, Product 17783, typically used?
Calcein-AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein-AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells.
Do the Scienceware® brand polypropylene products contain any leachable material?
Although Bel-Art does not specifically test their Scienceware® brand polypropylene products, they have no evidence of any problems with leachable material in the polypropylene.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Articles
Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
Related Content
Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
