P4864
Propidium iodide solution
≥94% purity, solution (1.0 mg/ml in water)
Synonym(s):
3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide
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About This Item
Recommended Products
Product Name
Propidium iodide solution,
Assay
≥94%
Quality Level
form
liquid
technique(s)
titration: suitable
color
faint orange to very dark orange, and Faint Red to Very Dark Red and Faint Purple to Very Dark Purple and Orange-Red and Red-Orange
solubility
water: 1 mg/mL
εmax
5400-6400 at 491-495 nm in phosphate buffer
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
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General description
Application
related product
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Certificates of Analysis (COA)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Does Propidium Iodide, Product P4864, stain viable or non-viable cells?
Propidium Iodide does not generally enter living cells, However it may be taken up by axonal terminals and retrogradely transported to neuronal cell bodies. Reference: Aschoff, A. and H. Hollander, Fluorescent compounds as retrograde tracers compared with horseradish peroxidase (HRP). I. A parametric study in the central visual system of the albino rat. J. Neurosci. Methods, 6(3), 179-197 (1982).
What are the excitation and emission maxima for Propidium Iodide, Product P4864?
Excitation maximum is at 493 nm in water. Emission maximum is at 636 nm in water
How is Propidium Iodide, Product P4864, used for Flow Cytometry?
Immediately before flow cytometric analysis, prepare a 0.5 mg/mL solution of propidium iodide by combining 50μL of 2X PBS with 50 μL of P4864. Add this solution to 1 mL of a cell suspension containing 10,000,000 cells. (Note: Cells exhibiting fluorescence above 630 nm should be excluded from further analysis.)
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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