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81845

Sigma-Aldrich

Propidium iodide

≥94% (HPLC)

Synonym(s):

3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide

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10 MG
CN¥650.36
25 MG
CN¥1,394.93
100 MG
CN¥2,699.25
500 MG
CN¥13,441.78

CN¥650.36

Available to ship onApril 17, 2025Details

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10 MG
CN¥650.36
25 MG
CN¥1,394.93
100 MG
CN¥2,699.25
500 MG
CN¥13,441.78

About This Item

Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
25535-16-4
Molecular Weight:
668.39
Beilstein:
3843838
EC Number:
247-081-0
MDL number:
MFCD00011921
UNSPSC Code:
12352200
PubChem Substance ID:
329769221
NACRES:
NA.77

CN¥650.36

Available to ship onApril 17, 2025Details

Request a Bulk Order

Quality Level

200

Assay

≥94% (HPLC)

form

powder

mp

220-225 °C (dec.) (lit.)

fluorescence

λex 482 nm; λem 608 nm (pH 7.2)
λex 540 nm; λem 608 nm (bound to DNA)

storage temp.

2-8°C

SMILES string

[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

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This Item
L4524L2637L6529
Lipopolysaccharides from Escherichia coli O55:B5 purified by phenol extraction

L2880

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O55:B5 purified by ion-exchange chromatography, TLR ligand tested

L4524

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O55:B5 purified by gel-filtration chromatography

L2637

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O55:B5 γ-irradiated, BioXtra, suitable for cell culture

L6529

Lipopolysaccharides from Escherichia coli O55:B5

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

300

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

solubility

H2O: 5 mg/mL, faintly hazy to hazy

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

PI is used for selective labelling of dead cells.[1]
Propidium iodide is a fluorescent intercalating agent used for staining.[2] It functions as an apoptosis marker[3] and has linear DNA-binding capacity.[4] Propidium iodide is an effective indicator of tissue injury.[5]

Application

Propidium iodide has been used:
  • to stain DNA in cell cycle analysis[6]
  • to stain cells[7] and cell nuclei[8][9]
  • for the preparation of propidium iodide (PI)stock solution[10]
  • to stain cells for flow cytometry[11][12]

in scrape-loading dye transfer assay[13]

Features and Benefits

This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H341

Hazard Classifications

Muta. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCK4543
    BCCH7010
    BCCF3528
    BCCC1398
    BCCB3059

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Intramolecular loop/tail interactions are essential for connexin 43-hemichannel activity
    Ponsaerts R, et al.
    Faseb Journal, 24(11), 4378-4395 (2010)
    Flow cytometry (2008)
    Effect of Different Combinations of the Growth Factors and Hormones on invitro Maturation of Goat Preantral Follicles
    Shankaraiah P, et al.
    International Journal of Current Microbiology and Applied Sciences, 7(4), 1956-1963 (2018)
    Intravital microscopic methods to evaluate anti-inflammatory effects and signaling mechanisms evoked by hydrogen sulfide
    Methods in Enzymology, 555, 93-125 (2015)
    High-throughput, non-perturbing quantification of lipid droplets with Digital Holographic Microscopy
    Campos V, et al.
    Journal of Lipid Research, jlr-D085217 (2018)
    View All Related Papers

    Articles

    Bioactive Molecules for Apoptosis

    Apoptosis regulation involves multiple pathways and molecules for cellular homeostasis.

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