Glutaraldehyde solution

Glutaraldehyde (Glutaric dialdehyde, Pentane-1,5-dial) is an aliphatic dialdehyde that can be employed as an effective biochemical reagent for various purposes such as a protein crosslinker, enzyme immobilization in microscopy, histochemistry, and cytochemistry. It serves as an amine-reactive homobifunctional crosslinker and a cell fixative before SDS-PAGE, staining, or electron microscopy, making it suitable for a wide range of morphological studies.

Glutaraldehyde is capable of efficiently crosslinking amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, in certain reactions, biotin hydrazides have been directly coupled to nucleic acids using glutaraldehyde, presenting a potential utility in conjugating fluorescent hydrazides and hydroxylamines to DNA. In histology studies, Glutaraldehyde is also employed to preserve tissue sections and prepare them for closer examination.

Cross-linking agent for gelatin,[1][2] poly(vinyl alcohol),[3] and polyheptapeptides.[4]

Glutaraldehyde may be used in the following studies:

• To compose the fixative solution (Glutaraldehyde + Paraformaldehyde + NaPO₄) for use in high-resolution light microscopy and electron microscopy studies.[5]
• To study the conjugation of goat anti-horseradish peroxidase with alkaline phosphatase by a reported method.[6]
• To compose the primary fixative, which is employed to protect the deterioration of cytoplasmic features of yeast cells during permanganate fixation.[7]

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