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10106399001

Roche

GTP

Name: GTP
Brand: ROCHE

>90% (HPLC), crystals, pkg of 250 mg

Synonym(s):

Guanosine 5′-triphosphate sodium salt hydrate, 5′-GTP-Na₂

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About This Item

Empirical Formula (Hill Notation):

C₁₀H₁₆N₅O₁₄P₃ · xNa⁺ · yH₂O

CAS Number:

36051-31-7

Molecular Weight:

523.18 (anhydrous free acid basis)

Beilstein:

4113439

MDL number:

MFCD00149227

UNSPSC Code:

41106305

PubChem Substance ID:

329748894

description

Disodium salt

Quality Level

100

Assay

>90% (HPLC)

form

crystals

mol wt

M_r 523.2 (GTP)
M_r 567.1 (GTP-Na₂)

packaging

pkg of 250 mg

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

SMILES string

O.O.[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1

InChI

1S/C10H16N5O14P3.2Na.2H2O/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;2*1H2/q;2*+1;;/p-2/t3-,5-,6-,9-;;;;/m1..../s1

InChI key

HPLNHYUZFVUXFJ-ZVQJTLEUSA-L

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Nucleic Acid Labeling

Roche Life Science Products

General description

GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA. GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome. It is also involved in polypeptide chain elongation. GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly.

GTP is supplied as the disodium salt in crystal form.

Application

GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents.

Quality

Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na⁺, 8% H2O

Preparation Note

Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3.

If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen.

Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A non-linear system patterns Rab5 GTPase on the membrane.

Alice Cezanne et al.

eLife, 9 (2020-06-09)

Proteins can self-organize into spatial patterns via non-linear dynamic interactions on cellular membranes. Modelling and simulations have shown that small GTPases can generate patterns by coupling guanine nucleotide exchange factors (GEF) to effectors, generating a positive feedback of GTPase activation

FtsZ polymerization assays: simple protocols and considerations.

Ewa Król et al.

Journal of visualized experiments : JoVE, (81)(81), e50844-e50844 (2013-12-05)

During bacterial cell division, the essential protein FtsZ assembles in the middle of the cell to form the so-called Z-ring. FtsZ polymerizes into long filaments in the presence of GTP in vitro, and polymerization is regulated by several accessory proteins.

The role of guanosine 5'-triphosphate in polypeptide chain elongation.

Y Kaziro

Biochimica et biophysica acta, 505(1), 95-127 (1978-09-21)

Marzia Govoni

Bio-protocol, 7(3), e2120-e2120 (2017-02-05)

In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs (mRNAs) and small RNAs, while RNA polymerase

Lehninger, A.L

Biochemistry, 316-316 (1977)

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