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Safety Information

1.04192

Sigma-Aldrich

Trypan Blue

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Synonym(s):
Direct blue 14
Linear Formula:
C34H24N6O14S4Na4
CAS Number:
72-57-1
Molecular Weight:
960.81
Colour Index Number:
23850
Beilstein:
4360496
MDL number:
MFCD00003969
NACRES:
NA.21

form

solid

Quality Level

200

potency

6200 mg/kg LD50, oral (Rat)

composition

Dye, ≥80% spectrophotometric assay

pH

9.8 (20 °C, 10 g/L in H2O)

mp

>300 °C (lit.)
>300 °C (lit.)

solubility

10 g/L

bulk density

800 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

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General description

Trypan blue (C.I. 23850) for microscopy, is a dry dye for the preparation of a color staining solution, staining histological sample material of human origin, e.g. trichrome staining in collagenous connective tissue. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Application

for microscopy

Analysis Note

Dye content (spectrophotometrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 590 - 600 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; water): 650 - 820
TLC-Test: passes test
Loss on drying (110 °C): ≤ 15 %
Suitability for microscopy: passes test

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Regulatory Information

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