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T6146

Sigma-Aldrich

Trypan Blue

powder, BioReagent, suitable for cell culture

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Synonym(s):
Direct blue 14, Niagara blue reagent, blue live/dead cell assay
Linear Formula:
C34H24N6O14S4Na4
CAS Number:
72-57-1
Molecular Weight:
960.81
Colour Index Number:
23850
Beilstein:
4360496
EC Number:
200-786-7
MDL number:
MFCD00003969
UNSPSC Code:
12352207
PubChem Substance ID:
57654686
NACRES:
NA.75

product line

BioReagent

Quality Level

300

form

powder

composition

Dye content, ~40%

packaging

glass bottle of 100 g
glass bottle of 25 g
glass bottle of 5 g

storage condition

dry at room temperature

technique(s)

cell culture | mammalian: suitable
tissue processing: suitable

mp

>300 °C (lit.)

solubility

H2O: 10 mg/mL

application(s)

cell analysis

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

human ... PTPN1(5770) , PTPRF(5792)

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General description

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.

Application

Trypan blue has been used:
  • in cell viability assay
  • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
  • to stain plant tissue in order to to visualize cell death

Biochem/physiol Actions

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Hazard Classifications

Carc. 1B

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Plant tissue trypan blue staining during phytopathogen infection.
Fernandez NB, et al.
Bio-protocol (2016)
A histological analysis of lens capsules stained with trypan blue for capsulorrhexis in phacoemulsification cataract surgery
Singh AJ, et al.
Eye (London, England), 17(5), 567-567 (2003)
Trypan blue exclusion test of cell viability
Strober W
Current Protocols in Immunology, 21(1), A-3B (1997)
Knockdown of PSMB7 induces autophagy in cardiomyocyte cultures: possible role in endoplasmic reticulum stress
Kyrychenko VO. et al.
Pathobiology, 81(1), 8-14 (2014)
Ulrich Putz et al.
Science signaling, 5(243), ra70-ra70 (2012-09-27)
Exosomes are microvesicles of endosomal origin that are secreted, and their contents (proteins, lipids, DNA, or microRNAs) can alter the physiological states of recipient cells. We demonstrated that phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor
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