1.11732
Trypan blue (C.I. 23850)
for microscopy
Synonym(s):
Trypan Blue, Benzamine blue, Tolidine-disazo-bis-8-amino-1-naphthol-3,6-disulfonic acid tetrasodium salt, 3,3′-Dimethyl-4,4′-bis(5-amino-4-hydroxy-2,7-disulfonaphthyl-3-azo)-[1,1′-biphenyl] tetrasodium salt, Direct blue 14
About This Item
Quality Level
form
solid
potency
6200 mg/kg LD50, oral (Rat)
composition
Dye, ≥80% spectrophotometric assay
pH
9.8 (20 °C, 10 g/L in H2O)
mp
>300 °C (lit.)
solubility
10 g/L
bulk density
800 kg/m3
εmax
650-820 at 590-600 nm in water at 0.01 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-30°C
SMILES string
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key
GLNADSQYFUSGOU-UHFFFAOYSA-J
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General description
The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.
Analysis Note
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 590 - 600 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; water): 650 - 820
TLC-Test: passes test
Loss on drying (110 °C): ≤ 15 %
Suitability for microscopy: passes test
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 1B
Storage Class Code
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
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