M9140
Methylene blue
Dye content, ≥82%, certified by the Biological Stain Commission, powder
Synonym(s):
Basic Blue 9, 3,7-bis(Dimethylamino)phenazathionium chloride, Tetramethylthionine chloride
About This Item
Recommended Products
Product Name
Methylene blue, certified by the Biological Stain Commission
grade
certified by the Biological Stain Commission
Quality Level
form
powder
composition
Dye content, ≥82%
technique(s)
microbe id | staining: suitable
color
dark green
solubility
H2O: 4mg/4ml
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CN(C)C1=CC=C2C(SC(C3=N2)=CC(C=C3)=[N+](C)C)=C1.[H]O[H].[Cl-]
InChI
1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
InChI key
WQVSELLRAGBDLX-UHFFFAOYSA-M
Gene Information
human ... CYP1A2(1544) , GSR(2936)
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General description
Application
- to stain vaginal cells
- for the preparation of protein aqueous ink
- to evaluate cell survival by staining
Biochem/physiol Actions
Suitability
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Certificates of Analysis (COA)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the solubility of Methylene blue, Product M9140?
The product is soluble in water at 50 mg/mL, EGME at 60 mg/mL and ethanol at 70 mg/mL. EGME is an abbreviation for ethylene glycol monomethyl ether, also known as 2-methoxyethanol or methyl cellosolve.
How can Methylene blue, Product M9140, be used?
Probably its most important application is as an in vitro diagnostic bacteriology, cytology, hematology, and histology stain. Methylene Blue serves as a precursor to the Methylene Azures and as a component of the various Ramanowsky-type blood stains (Wright, Leishman, Giemsa and others). While pure Methylene Blue is not a good nuclear stain, it readily undergoes oxidative demethylation to form the various Methylene Azures, which are good nuclear stains.
How is dye content determined for Methylene blue, Product M9140?
Dye content is determined spectrophotometrically by noting the dye's absorbance peak, which is typically near 665 nm, or by calculating the ratio of the absorbances at 650 nm over 680 nm. (The dye exhibits smaller peaks at approximately 292 and 246 nm).
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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