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T3260

Sigma-Aldrich

Toluidine Blue O

Powder

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Synonym(s):
Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
92-31-9
Molecular Weight:
305.83
Colour Index Number:
52040
EC Number:
202-146-2
MDL number:
MFCD00011934
UNSPSC Code:
12171500
PubChem Substance ID:
24900122
NACRES:
NA.47

grade

technical grade

Quality Level

200

form

powder

composition

Dye content, ~80%

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

InChI

1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H

InChI key

GEDVVYWLPUPJJZ-UHFFFAOYSA-N

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General description

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. It is a metachromatic dye suitable for a wide variety of histological staining procedures.
A metachromatic dye suitable for a wide variety of histological staining procedures.

Application

Toluidine Blue O has been used to stain a variety of specimens like mesenchymal cells, taste buds, osteoblasts, matrix, etc.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is there a difference between Toluidine Blue O and Toluidine Blue and which is best for staining tissue sections?

    Toluidine Blue O and Toluidine Blue are generally regarded as synonyms. The dye can be found in 2 forms. There is a chloride form and zinc chloride double salt.  Products T3260 and 198161 are the chloride form. This is the form certified by the Biological Stain Commission. The dye listed as simply Toluidine Blue, Product 89640, is the zinc chloride double salt. For routine use today, the chloride form should be utilized. The product number recommended for biological/histological staining is Product 198161, Toluidine Blue O.  This product has been found to provide acceptable staining of tissue by the Biological Stain Commission. Products 198161 and 89640 have not been suitability tested for the staining of tissue sections.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Polychromatic staining of plant cell walls by toluidine blue O
T.P.O'Brien
Protoplasma, 59(2) (1964)
An investigation of cellular dynamics during the development of intramembranous bones: the scleral ossicles
J. Jabalee
Journal of Anatomy (2013)
Mrinalini Sharma et al.
Antimicrobial agents and chemotherapy, 52(1), 299-305 (2007-10-31)
Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed
The canine epiphyseal-derived mesenchymal stem cells are comparable to bone
marrow derived-mesenchymal stem cells
Ya-Pei CHANG
Surgery (2015)
Fibroblast-derived matrix (FDM) as a novel vascular endothelial growth factor delivery platform
Ping Du
Journal of Controlled Release : Official Journal of the Controlled Release Society (2014)
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