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安全信息

ECM001

Sigma-Aldrich

Human Fibronectin

recombinant, expressed in HEK 293 cells, powder, suitable for cell culture

别名:

纤连蛋白 溶液 来源于人类成纤维细胞, CIG, FN, 冷不溶性球蛋白

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About This Item

MDL编号:
MFCD00131062
UNSPSC代码:
12352202
NACRES:
NA.75

产品名称

人纤维连接蛋白, recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

生物来源

human

质量水平

200

重组

expressed in HEK 293 cells

无菌性

sterile

方案

≥95% (SDS-PAGE)

表单

lyophilized powder

分子量

259.5 kDa (The protein migrates as a higher band on SDS-PAGE due to glycosylation.)

包装

pkg of 200 μg

技术

cell culture | mammalian: suitable

杂质

≤1 EU/μg endotoxin, tested

溶解性

water: soluble

NCBI登记号

NM_212482.4

UniProt登记号

P02751

运输

ambient

储存温度

−20°C

基因信息

human ... FN1(2335)

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相关类别

一般描述

纤连蛋白（FN）是糖蛋白，5％-9％的糖类以 N-和 O-连接寡糖的形式存在。 属于配体糖蛋白家族。FN基因位于人类染色体2q35。FN以两种主要的同工型存在，即，基于血浆的可溶形式和基于细胞外基质（ECM）的不溶形式。纤连蛋白结构包含FNI、FNII和FNIII重复序列的重复单元。它还具有血纤蛋白结合位点，以二聚体形式存在。FN1基因的可变剪接导致产生许多同工型。该纤连蛋白是用全人类生产系统在人细胞中生产，不含血清。该人类细胞表达系统可实现与人类蛋白相仿的糖基化和折叠，通常可为培养物中的蛋白提供更好的稳定性支持。

应用

上皮细胞，间充质细胞，神经元细胞，成纤维细胞，神经嵴细胞，内皮细胞。
推荐用作细胞培养基质，1-5μg/cm 2 或 0.5-50μg/ml。最佳浓度取决于细胞类型以及应用或研究目的。
人纤连蛋白用于包被细胞培养板，培养内皮祖细胞。它还作为Dulbecco′s改良Eagle′培养基（DMEM）成分，用于胶质母细胞瘤细胞的 体外 人工基膜侵袭试验。

生化/生理作用

纤连蛋白（FN）对胚胎发育至关重要，介导细胞的粘附和迁移功能。介导鼻咽癌（NPC）细胞凋亡。血浆FN调节血栓形成。纤连蛋白可作为整联蛋白受体家族 的配体，包含胶原蛋白、糖蛋白和糖胺聚糖结合位点。 FNI基因突变造成纤连蛋白的可溶形式和不可溶形式失衡，导致遗传性肾脏疾病-肾小球病中的肾小球沉积。FN1基因的错义突变与骨骼发育不良疾病-脊柱干骺端发育不良有关。

特点和优势

  • 人源纤连蛋白
  • 通过HEK 293细胞中的重组表达生产
  • 内毒素水平低
  • 无细菌污染

外形

以粉末形式提供，由盖缓冲盐水冻干。

分析说明

通过测定固定化纤维连接蛋白支持 CHO 细胞黏附的能力，检测重组人纤连蛋白在培养中的生物活性。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000233596
0000233596
0000154101
0000106218

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Jeannette Huaman et al.
Cells, 9(7) (2020-07-08)
Fibronectin (FN1) is an extracellular matrix protein gaining increasing attention for its multifaceted roles in cancer progression. Using our recently established circulating tumor cell (CTC) lines, we had demonstrated increased FN1 expression and enhanced migration in CTC lines, in comparison
Anna Lemańska-Perek et al.
Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 28(11), 1561-1567 (2019-10-10)
Fibronectin (FN) is a widely distributed glycoprotein which is present in different bodily fluids, on the surface of cells and in the extracellular matrix (ECM). It plays roles in various processes, including cell adhesion, migration, growth, proliferation, and tissue repair.
Hsiang-Chi Tseng et al.
Nature communications, 11(1), 4810-4810 (2020-09-25)
Chimeric antigen receptor (CAR) therapy is a promising immunotherapeutic strategy for treating multiple refractory blood cancers, but further advances are required for solid tumor CAR therapy. One challenge is identifying a safe and effective tumor antigen. Here, we devise a
The association between hyperhomocysteinemia and cerebral small vessel disease
Yang Y, et al.
International Journal of Clinical and Experimental Medicine, 12, 13877-13883 (2019)
Jinting Wang et al.
American journal of translational research, 9(10), 4502-4511 (2017-11-10)
Fibronectin 1 (FN1) is a member of the glycoprotein family located on chromosome 2q35. It has been reported that FN1 is upregulated in many tumors, and its expression is negatively related to the prognosis and survival of cancer patients. Through
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