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F2006

Sigma-Aldrich

Human Fibronectin

from human plasma, powder, suitable for cell culture

Synonym(s):

Fibronectin

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About This Item

EC Number:
289-149-2
MDL number:
MFCD00131062
UNSPSC Code:
12352202
eCl@ss:
42020141
NACRES:
NA.75

product name

Fibronectin human plasma, lyophilized powder, BioReagent, suitable for cell culture

biological source

human plasma

Quality Level

200

sterility

sterile

product line

BioReagent

Assay

≥85% (SDS-PAGE)

form

lyophilized powder

mol wt

450 kDa

packaging

pkg of 5 x 5 mg
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

concentration

>1 mg/mL protein

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

impurities

HBSAG, none detected
 HCV, none detected
 HIV-1/HIV-2, none detected

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI accession no.

NM_212482.4

UniProt accession no.

P02751

Binding Specificity

Peptide Source: Collagen

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FN1(2335)

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General description

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous in nature and form functional unit arranged linearly on two subunit arms. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of fibronectin protein due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

Application

Fibronectin from human plasma is used for the following applications:
  • Used in cell seeding and neuronal differentiation (as one of the components in expansion medium for the re-suspension of CBSCs (Cord blood stem cells) and is also used in differentiation medium)
  • Used in cell culture (the culture chamber was coated with fibronectin for the expression of HEK293 cells)
  • In-vitro fibronectin (FN) pulldown assays
  • Protein specificity
  • Used in cell migration study

Biochem/physiol Actions

Fibronectin (FN) plays an essential role in multiple biological functions such as, angiogenesis, cell migration cell-to-cell adhesion, cell-to-basement-membrane attachment, clot stabilization, embryogenesis, differentiation, wound healing, as well as binding of pathogen to mammalian cells and extracellular matrix. Studies in Chinese Han population show that mutations in this gene might be linked with susceptibility to knee osteoarthritis (OA). Splice variant extra domain-B (EDB) of FN is a hallmark of tumor angiogenesis, and with increased matrix stiffness there is an increase in the production of this isoform by endothelial cells. In colorectal cancer (CRC), EDA is linked with metastasis, vasculogenesis and tumorigenesis and promotes epithelial-mesenchymal transition.

Caution

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B, hepatitis C and syphilis. In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I choose which fibronectin product to use to coat culture surfaces for cell growth?

    The species source for the fibronectin used when coating culture surfaces is not important.  It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.

  4. How long can I store the fibronectin solution?

    The product is good for at least a year at 2-8 °C if kept sterile.

  5. How long can I store fibronectin coated plates, etc?

    Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.

  6. What is the procedure for coating culture surfaces with fibronectin?

    To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume.  2. Allow to air dry for at least 45 minutes at room temperature.  Excess fibronectin may be removed by aspiration, but this is not necessary.

  7. How do I solubilized Fibronectin?

    Dissolve at 1 mg/mL in water. Allow to dissolve for at least 30 minutes at 37 °C. A small amount of undissolved material may remain. This will not affect product performance.

  8. How do I test the wells/flasks that have been coated with fibronectin?

    We test the coated culture surface by culturing cells.  We compare the adherence of the cells to wells that have been coated with media containing BSA (negative) and media containing 10% FBS (positive).  The cell growth should be not less than 50% of the positive control and greater than 50% of the negative control.

  9. How do I determine how much fibronectin is in solution?

    As the product may contain undissolved material, the amount of fibronectin in solution can be determined by UV absorbance.  The extinction coefficient for a 1% solution (10 mg/ml) is 13.5 at 280 nm.

  10. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  11. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  12. What is the difference between Fibronection Products F2006 and F1056?

    The difference between Product Nos. F2006 and F1056 is in the purity specifications and endotoxin testing.  Product  No. F2006 has a purity specification of ≥90% and is not tested for endotoxin contamination.  Product No. F1056 has a purity specification of ≥95% and is tested for endotoxin contamination with a specification of ≤100 EU/mg.

  13. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Fibronectin: a brief overview of its structure, function, and physiology
Proctor R A, et al.
Reviews of Infectious Diseases, 4, S317-S321 (1987)
Timothy E Quan et al.
Methods in molecular medicine, 135, 423-434 (2007-10-24)
Fibrocytes circulate in the peripheral blood, produce collagen and other matrix proteins, and express cell surface markers indicative of a hematopoetic origin distinguishing them from fibroblasts. Circulating fibrocytes were first identified in 1994 in a model system of wound repair
Marcin Jurga et al.
Biomaterials, 32(13), 3423-3434 (2011-02-18)
Currently, there are no effective therapies to restore lost brain neurons, although rapid progress in stem cell biology and biomaterials development provides new tools for regeneration of central nervous system. Here we describe neurogenic properties of bioactive scaffolds generated by
Kerim B Kaylan et al.
Integrative biology : quantitative biosciences from nano to macro, 8(12), 1221-1231 (2016-11-02)
Carcinoma progression is influenced by interactions between epithelial tumor cells and components of their microenvironment. In particular, cell-extracellular matrix (ECM) interactions are known to drive tumor growth, metastatic potential, and sensitivity or resistance to therapy. Yet the intrinsic complexity of
Marianne J van den Heuvel et al.
Journal of virology, 79(13), 8164-8170 (2005-06-16)
Bovine leukemia virus (BLV) is a deltaretrovirus that infects cattle worldwide. In agriculturally intensive regions, approximately 30% of dairy cows are BLV infected. Like the human T-cell leukemia virus (HTLV), there is a lengthy period of viral quiescence after initial
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Fibronectin

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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