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Safety Information

10838039001

Roche

Fibronectin

from human plasma

Synonym(s):

Fibronectin

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0.5 MG
CN¥4,968.26

CN¥4,968.26

Available to ship onApril 17, 2025Details

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0.5 MG
CN¥4,968.26

About This Item

MDL number:
MFCD00131062
UNSPSC Code:
12352207

CN¥4,968.26

Available to ship onApril 17, 2025Details

biological source

human plasma

Quality Level

100

sterility

non-sterile; 0.2 μm filtered

Assay

95% (SDS-PAGE)

form

lyophilized (clear, colorless solution after reconstitution)

mol wt

440 kDa

packaging

pkg of 5 mg

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

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1 of 4

This Item
F2006F0287ECM001
Fibronectin from human plasma

10838039001

Fibronectin

Fibronectin human plasma lyophilized powder, BioReagent, suitable for cell culture

F2006

Fibronectin human plasma

Fibronectin Proteolytic Fragment from human plasma lyophilized powder, 70 kDa

F0287

Fibronectin Proteolytic Fragment from human plasma

Fibronectin human recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

ECM001

Fibronectin human

sterility

non-sterile; 0.2 μm filtered

sterility

sterile

sterility

-

sterility

sterile

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

assay

95% (SDS-PAGE)

assay

≥85% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized (clear, colorless solution after reconstitution)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

General description

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.[1]

Specificity

Active on most mammalian cells

Application

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.[2][3][4]

Testing Method

Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)

Physical form

Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.

Preparation Note

Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstitution

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    72438200
    67434100
    66091400
    60026000
    57114400

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    Daniella Penko et al.
    Cell transplantation, 24(1), 37-48 (2013-09-28)
    The success of pancreatic islet transplantation is limited by delayed engraftment and suboptimal function in the longer term. Endothelial progenitor cells (EPCs) represent a potential cellular therapy that may improve the engraftment of transplanted pancreatic islets. In addition, EPCs may
    Hao Wang et al.
    Microsystems & nanoengineering, 8, 49-49 (2022-05-14)
    Cardiovascular disease is the number one cause of death in humans. Therefore, cardiotoxicity is one of the most important adverse effects assessed by arrhythmia recognition in drug development. Recently, cell-based techniques developed for arrhythmia recognition primarily employ linear methods such
    Ana Lonic et al.
    STAR protocols, 3(2), 101305-101305 (2022-05-03)
    Previously published protocols for quantification of endosomal recycling are limited by the use of radioactive reagents, washing of cells in reducing buffers, or the requirement for large numbers of cells. Here, we describe a protocol for quantification of endosomal recycling
    Daniella Penko et al.
    Islets, 3(3), 73-79 (2011-04-12)
    Pancreatic islet transplantation is limited by extensive apoptosis and suboptimal function of the implanted islets in the longer term. Endothelial progenitor cells (EPC) may be ideal for enhancing both the survival and function of transplanted islets. Here, we describe for
    Weiwei Wang et al.
    Journal of oncology, 2022, 4499876-4499876 (2022-07-09)
    Application of extracellular vesicles (EVs) for cancer treatment has been well-documented. We probed into the potential role of cervical cancer cells-secreted EVs by transferring miR-146a-5p in cervical cancer. After characterization of miR-146a-5p expression in clinical cervical cancer tissue samples, gain-
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    Protocols

    Fibronectin Coating Protocol

    Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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