Skip to Content
Merck
CN

F2518

Human Fibronectin

from human foreskin fibroblasts, powder, suitable for cell culture

Synonym(s):

Fibronectin

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352202
NACRES:
NA.75
EC Number:
289-149-2
MDL number:
MFCD00131062

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Fibronectin human foreskin fibroblasts, lyophilized powder, suitable for cell culture

biological source

human foreskin fibroblasts

product line

BioReagent

form

lyophilized powder

mol wt

~550 kDa

packaging

pkg of 0.5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

impurities

Human Source, none detected (HCV)
 Human Source, none detected (HIV)
 Human Source, none detected (HTLV)
 Human Source, none detected (HbSAg)
 Microbial Contamination, passes test

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI accession no.

NM_212482.4

UniProt accession no.

P02751

binding specificity

Peptide Source: Collagen

shipped in

ambient

storage temp.

−20°C

Quality Level

200

Gene Information

human ... FN1(2335)

Looking for similar products? Visit Product Comparison Guide

Related Categories

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Application

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells
Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

Disclaimer

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Features and Benefits

  • Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
  • It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
  • The starting material is tested and found negative for HIV and HBsAg.
  • The product is soluble in sterile water, yielding a clear, colorless solution.
  • It can be stored at -20°C for at least three years.

General description

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.

Physical form

Lyophilized in CAPS buffered saline

Preparation Note

Store reconstituted solution in working aliquots at -20°C or lower.
dialyzed against chloroform

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000420443
0000420443
0000218931
0000218931
0000129869

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery.
Tsang T M, et al.
Infection and Immunity, 78(8), 3358-3368 (2010)
Regulation of matrix Accumulation (2012)
Fibronectin
Fabrizio M and Angela S
Encyclopedia of Immunology, 909-913 (1998)
Natural variant of collagen-like protein A in serotype M3 group A Streptococcus increases adherence and decreases invasive potential.
Flores A R, et al.
Infection and Immunity (2015)
Ramesh B Kasetti et al.
Scientific reports, 7(1), 14951-14951 (2017-11-04)
Increased synthesis and deposition of extracellular matrix (ECM) proteins in the trabecular meshwork (TM) is associated with TM dysfunction and intraocular pressure (IOP) elevation in glaucoma. However, it is not understood how ECM accumulation leads to TM dysfunction and IOP
View All Related Papers

Articles

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Fibronectin

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

Fibronectin

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

See All

Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Related Content

Data Sheet - F2518

Instructions

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service