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Key Documents

N9784

Sigma-Aldrich

Neutralization Solution for Blood

sufficient for 100 reactions, Molecular Biology, sufficient for 1000 reactions

Synonym(s):

DNA neutralization solution, Neutralization buffer DNA extraction

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Select a Size

5 G
CN¥339.62
25 G
CN¥1,109.20
100 G
CN¥2,575.97

CN¥339.62


Available to ship onApril 27, 2025Details



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5 G
CN¥339.62
25 G
CN¥1,109.20
100 G
CN¥2,575.97

About This Item

UNSPSC Code:
12352200
NACRES:
NA.25

CN¥339.62


Available to ship onApril 27, 2025Details


grade

Molecular Biology

Quality Level

form

liquid

usage

sufficient for 100 reactions
sufficient for 1000 reactions

storage temp.

room temp

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This Item
B9754B7535B4429
assay

≥99.0% (titration)

assay

≥98.0% (titration)

assay

≥98.0% (titration)

assay

≥98.0%

Quality Level

200

Quality Level

400

Quality Level

400

Quality Level

400

form

crystalline powder

form

crystalline powder

form

powder

form

crystalline powder

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing
general analytical
microbiology

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

solubility

water: 0.5 g/mL, clear, colorless to very faintly yellow

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

H2O: 1 M, clear, colorless

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

General description

Neutralization Solution for Blood is used along with lysis solution for blood (L3289) for the rapid extraction and neutralization of human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

This solution is a component of the Extract-N-Amp and the REDExtract-N-Amp Blood PCR Kits

Application

Neutralization Solution for blood has been used to neutralize extracted DNA prior to polymerase chain reaction (PCR) amplification.[1][2][3][4][5]

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Martina Veith et al.
International journal of chronic obstructive pulmonary disease, 14, 2535-2542 (2019-12-11)
Alpha-1-antitrypsin deficiency (AATD) is a rare hereditary condition resulting from the mutations in the SERPINA1 (serine protease inhibitor) gene and is characterized by low circulating levels of the alpha-1 antitrypsin (AAT) protein. The traditional algorithm for laboratory testing of AATD
Sabrina M Rodriguez et al.
The Journal of general virology, 90(Pt 11), 2788-2797 (2009-07-10)
Previous studies have classified the env sequences of bovine leukemia virus (BLV) provirus from different locations worldwide into between two and four genetic groupings. These different studies gave unique names to the identified groups and no study has yet integrated
Amber K Bowers et al.
Genetics, 164(4), 1345-1353 (2003-08-22)
To take advantage of available expressed sequence tags and genomic sequence, we have developed 64 PCR-based molecular markers in Chlamydomonas reinhardtii that map to the 17 linkage groups. These markers will allow the rapid association of a candidate gene sequence
G Henderson et al.
Archives of disease in childhood. Fetal and neonatal edition, 94(2), F124-F128 (2007-09-05)
The inflammatory cytokine cascade is implicated in the pathogenesis of necrotising enterocolitis (NEC). Genetic association studies of cytokine polymorphisms may help to detect molecular mechanisms that are causally related to the disease process. To examine associations between the common genetic
Leandro S Thiago et al.
Haematologica, 99(1), 155-162 (2013-07-23)
The B-cell compartment in which multiple myeloma stem cells reside remains unclear. We investigated the potential presence of mature, surface-membrane immunoglobulin-positive B lymphocytes clonally related to the tumor bone marrow plasma cells among different subsets of peripheral blood B cells

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