Validated Non-Human Cell Types for Millicell^® DCI Digital Cell Imager

CHO-K1 Cell Line

1. Catalog number: 85051005
2. Source: Non-human; hamster ovary
3. Cell type/morphology: Adherent, epithelial
4. Application: Nutritional and gene expression studies have been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B), and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
   Ham′s F12 (N4888)+ 2mM Glutamine (G7513)+ 10% Fetal Bovine Serum FBS/FCS (ES-009-B).
   Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 1-2 x 10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.
10. Protocols: Cell culture protocols are available in the product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter using a 60 µm sensor.

CNS-1 Cell Line

1. Catalog number: SCC487
2. Source: Rat brain tumor
3. Cell type/morphology: Adherent, glial
4. Application: As a model for glioma immunotherapies
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and 10X image with confluency mask (B)

8. Cell culture media: Cells were cultured in RPMI (R0883) with 10% FBS (ES-009-B) and 1% L-Glutamine (TMS-002-C).
9. Protocols: Cell culture protocols are available in product datasheet.
10. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter using a 60 µm sensor.

H9C2 Cell Line

1. Catalog number: 88092904
2. Source: Non-human; rat cardiovascular
3. Cell type/morphology: Adherent, myoblast
4. Application: Differentiation studies
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B), and the 10X image with confluency mask (C).

8. Growth curve:

9. Cell culture media:
   DMEM-Hi-glucose medium (D6429) + 2 mM L-Glutamine (G7513) + 10% FBS (ES-009-B).
   Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e. seeding 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Important to passage at sub-confluence, and re-clone periodically to keep differentiated characteristics. Cells will fuse to form myotubes if allowed to reach confluency.
10. Protocols: Cell culture protocols are available in the product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

J774A.1 Cell Line

1. Catalog number: 91051511
2. Source: Non-human; mouse blood
3. Cell type/morphology: Semi-adherent
4. Application: Metabolic studies, test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycosides isolated from Delphinium sps.
5. Biosafety level: 2
6. Handling: Medium
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B), and the 10X image with confluency mask (C).

8. Growth curve:

9. Cell culture media:
   DMEM-Hi-glucose medium (D6429) + 2 mM L-Glutamine (G7513) + 10% FBS (ES-009-B).
   Cells are subcultured by harvesting attached and suspension cells. Remove the majority of the medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio, i.e. seeding at 1-3 x 10,000 cells/cm² Incubate at 37°C; 5% CO₂. On receipt, growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10⁴/cm and should begin to show signs of re-attachment within 48 hours.
10. Protocols: Cell culture protocols are available in the product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

L929 Cell Line

1. Catalog number: 85011425
2. Source: Mouse adipose tissue
3. Cell type/morphology: Adherent, fibroblast
4. Application: Virus studies: PRV, VSV (Indiana), HSV
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth curve:

9. Cell culture media:
   DMEM-Hi-glucose medium (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS (F2442).
   Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 5 x 1,000-2 X 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.

MDCK Cell Line

1. Catalog number: 84121903
2. Source: Non-human, Canine kidney
3. Cell type/morphology: Adherent, epithelial
4. Application: Virus studies: SVEV, VSV, Vaccinia, adeno, and reoviruses
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B), and the 10X image with confluency mask (C).

8. Growth curve:

9. Cell culture media:
   EMEM (EBSS)(M2279) + 2 mM Glutamine (TMS-002-C) + 1% Non-Essential Amino Acids (NEAA)(TMS-001-C) + 10% Fetal Bovine Serum (FBS)(ES-009-B).
   Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in the product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

Oli-neu Cell Line

1. Catalog number: SCC261
2. Source: Non-human, mouse
3. Cell type/morphology: Adherent
4. Application: neuronal physiology, oligodendrocyte biology
5. Biosafety level: 2
6. Handling: Advanced
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 10X image with confluency mask (B), differentiated Oli-neu oligodendrocytes (C)

8. Genetic profile:

9. Cell culture media:
   Cells were thawed and expanded in Oli-neu Expansion Medium in poly-L-lysine-coated tissue culture flasks. Oligo-dendrocyte differentiation was done in expansion medium with daily addition of 1 mM dbcAMP (D0627)
10. Protocols: Cell culture protocols are available in the product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

OSUMMER.1 Cell Line

1. Catalog number: SCC445
2. Source: Non-human, male mouse
3. Cell type/morphology: Adherent
4. Application: Melanoma specific to NRAS mutation Q61R
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and the 10X image with confluency mask (B).

8. Genetic profile:

9. Cell culture media:
   Cells were thawed and expanded in OSUMMER Expansion Medium comprised of DMEM-High Glucose medium (D5796) with 10% FBS (ES-009-B), 2 mM L-Glutamine (TMS-002-C), and 1X penicillin/streptomycin (TMS-AB2-C, optional).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

OSUMMER.2 Cell Line

1. Catalog number: SCC446
2. Source: Non-human, mouse
3. Cell type/morphology: Adherent
4. Application: Melanoma specific to NRAS mutation Q61R
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and the 10X image with confluency mask (B).

8. Genetic profile:

9. Cell culture media:
   Cells were thawed and expanded in OSUMMER Expansion Medium comprised of DMEM-High Glucose medium (D5796) with 10% FBS (ES-009-B), 2 mM L-Glutamine (TMS-002-C), and 1X penicillin/streptomycin (TMS-AB2-C, optional).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

OSUMMER.3 Cell Line

1. Catalog number: SCC447
2. Source: Non-human, female mouse
3. Cell type/morphology: Adherent
4. Application: Melanoma specific to NRAS mutation Q61R
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and the 10X image with confluency mask (B).

8. Genetic profile:

9. Cell culture media:
   Cells were thawed and expanded in OSUMMER Expansion Medium comprised of DMEM-High Glucose medium (D5796) with 10% FBS (ES-009-B), 2 mM L-Glutamine (TMS-002-C), and 1X penicillin/streptomycin (TMS-AB2-C, optional).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

OSUMMER.4 Cell Line

1. Catalog number: SCC448
2. Source: Male mouse, melanoma
3. Cell type/morphology: Adherent, epithelial
4. Application: Carries the NRAS mutation Q61R and may be used to create syngeneic mouse melanoma models
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and the 10X image with confluency mask (B).

8. Genetic profile:

9. Cell culture media: Cells were cultured in DMEM (D5796) + 10% FBS (ES-009-B) + 1% L-Glutamine (TMS-002-C).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter using a 60 µm sensor.

OSUMMER.6 Cell Line

1. Catalog number: SCC450
2. Source: Male mouse, melanoma
3. Cell type/morphology: Adherent, epithelial
4. Application: Carries the NRAS mutation Q61R and may be used to create syngeneic mouse melanoma models
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and the 10X image with confluency mask (B).

8. Genetic profile:

9. Cell culture media: Cells were cultured in DMEM (D5796) + 10% FBS (ES-009-B) + 1% L-Glutamine (TMS-002-C).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter using a 60 µm sensor.

OSUMMER.7 Cell Line

1. Catalog number: SCC451
2. Source: Male mouse, melanoma
3. Cell type/morphology: Adherent, epithelial
4. Application: Carries the NRAS mutation Q61R and may be used to create syngeneic mouse melanoma models
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and the 10X image with confluency mask (B).

8. Genetic profile:

9. Cell culture media: Cells were cultured in DMEM (D5796) + 10% FBS (ES-009-B) + 1% L-Glutamine (TMS-002-C).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter using a 60 µm sensor.

OSUMMER.8 Cell Line

1. Catalog number: SCC452
2. Source: Non-human, female mouse
3. Cell type/morphology: Adherent
4. Application: Melanoma specific to NRAS mutation Q61K
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and the 10X image with confluency mask (B).

8. Genetic profile:

9. Cell culture media:
   Cells were thawed and expanded in OSUMMER Expansion Medium comprised of DMEM-High Glucose medium (D5796) with 10% FBS (ES-009-B), 2 mM L-Glutamine (TMS-002-C), and 1X penicillin/streptomycin (TMS-AB2-C, optional).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

OSUMMER.9 Cell Line

1. Catalog number: SCC453
2. Source: Male mouse, melanoma
3. Cell type/morphology: Adherent, epithelial
4. Application: Carries the NRAS mutation Q61L and may be used to create syngeneic mouse melanoma models
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and the 10X image with confluency mask (B).

8. Genetic profile:

9. Cell culture media: Cells were cultured in DMEM (D5796) + 10% FBS (ES-009-B) + 1% L-Glutamine (TMS-002-C).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter using a 60 µm sensor.

OSUMMER.10 Cell Line

1. Catalog number: SCC454
2. Source: Non-human, female mouse
3. Cell type/morphology: Adherent
4. Application: Melanoma specific to NRAS mutation Q13R
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (B) and the 10X image with confluency mask (A).

8. Genetic profile:

9. Cell culture media:
   Cells were thawed and expanded in OSUMMER Expansion Medium comprised of DMEM-High Glucose medium (D5796) with 10% FBS (ES-009-B), 2 mM L-Glutamine (TMS-002-C), and 1X penicillin/streptomycin (TMS-AB2-C, optional).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

OSUMMER.11 Cell Line

1. Catalog number: SCC455
2. Source: Non-human, female mouse
3. Cell type/morphology: Adherent
4. Application: Melanoma specific to NRAS mutation Q61R
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and the 10X image with confluency mask (B).

8. Genetic profile:

9. Cell culture media:
   Cells were thawed and expanded in OSUMMER Expansion Medium comprised of DMEM-High Glucose medium (D5796) with 10% FBS (ES-009-B), 2 mM L-Glutamine (TMS-002-C), and 1X penicillin/streptomycin (TMS-AB2-C, optional).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

SCP2 Cell Line

1. Catalog number: SCC458
2. Source: Non-human, mouse
3. Cell type/morphology: Adherent, epithelial
4. Application: Mammary epithelium biology
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and the 10X image with confluency mask (B).

8. Genetic profile:

9. Cell culture media:
   Cells were thawed and expanded in Scp2 Expansion Medium comprised of DMEM/F12-High medium (D8062) with 2% FBS (ES-009-B), 2 mM L-Glutamine (TMS-002-C), 5 mg/mL insulin (I9278), and 1X penicillin/streptomycin (TMS-AB2-C, optional).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

Vero Cell Line

1. Catalog number: 84113001
2. Source: Non-human, African Green Monkey
3. Cell type/morphology: Adherent, fibroblast-like kidney cell
4. Application: Media testing, Toxicology, Bioproduction, 3D cell culture
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
   DMEM-Hi-glucose medium (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS (F2442).
   Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted using a Scepter™ 3.0 automated cell counter.

Vero C1008 Cell Line

1. Catalog number: 85020206
2. Source: Non-human, African Green Monkey [Vero 76, clone E6, Vero E6]
3. Cell type/morphology: Adherent, fibroblast-like kidney cell
4. Application: Media testing, Toxicology, Bioproduction, 3D cell culture
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
   DMEM-Hi-glucose medium (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS (F2442).
   Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were diluted 1:16 and counted using a Scepter™ 3.0 automated cell counter using 60 µm sensor.