Validated Human Cell Types for Millicell^® DCI Digital Cell Imager

A431 Cell Line

1. Catalog number: 85090402
2. Source: Human skin (female), squamous carcinoma
3. Cell type/morphology: Adherent, epithelial
4. Application: To study p38 MAPK-induced apoptosis, to study melanoma-specific CD44 receptor, to study the effect of anti-cancer medical plants
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
EMEM (EBSS) + 2 mM L-Glutamine (G7513) + 1% Non-Essential Amino Acids (NEAA)  + 10% FBS (F2442).
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4 x 10,0000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.

A549 Cell Line

1. Catalog number: 86012804
2. Source: Human, lung (carcinoma)
3. Cell type/morphology: Adherent, epithelial
4. Application: To study the role of BChTT enzyme in cancer inhibition, to study the effects of insulin and IGF1, to study alveolar type II differentiation
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
Ham’s F12K medium + 10% FBS (F2442).
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4 x 10,0000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.

ALMC-1 Cell Line

1. Catalog number: SCC430
2. Source: Human bone marrow
3. Cell type/morphology: Suspension cell line
4. Application: Plasma cell myeloma/amyloidosis
5. Biosafety level: 2
6. Handling: Medium
7. Images: Brightfield image on Millicell^® DCI (A) and flow cytometry histogram showing expression of CD44 in yellow and unstained cells in gray (B)

8. Genetic profile:

9. Cell culture media:
   Cells were thawed in ALMC-1 recovery medium and expanded in ALMC-1 Expansion Medium comprised of IMDM medium (I6529) with 10% FBS (ES-009-M), 2 mM L-Glutamine (TMS-002-C), 2 ng/mL IL-6 (GF338) and 1X penicillin/streptomycin (TMS-AB2-C, optional).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

Caco-2 Cell Line

1. Catalog number: 86010202
2. Source: Human colon (Caucasian colon adenocarcinoma)
3. Cell type/morphology: Adherent, epithelial
4. Application: To study if components of the sigma B regulon in Listeria monocytogenes contribute to cell invasion, for functional analysis of bacteriocin divercin AS7, to study the intracellular invasion by Listeria ivanovii
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B), and the 10X image with confluency mask (C).

8. Growth curve:

9. Cell culture media:
   EMEM (EBSS) (M2279) + 2 mM Glutamine (G7513) + 1% Non-Essential Amino Acids (NEAA) (M7145) + 10% Fetal Bovine Serum (FBS) (ES-009-M).
   Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. During routine subculture, the cells should always be subcultured before they achieve confluence. Cells may show the appearance of circular vacuoles in the cytoplasm. These may increase in frequency as the culture density increases to confluence. To reduce their frequency, media change confluent cultures after 2-3 days if not subcultured. Cells can clump if not separated into a single-cell suspension when split.
10. Protocols: Cell culture protocols are available in the product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

HCT116 Cell Line

1. Catalog number: 91091005
2. Source: Human colon carcinoma
3. Cell type/morphology: Adherent, epithelial-like
4. Application: Tumorigenicity studies; to study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA); to study the uptake of fluorescently-labelled siRNA; to study the effect of polyamine transport system in the selective uptake of Quilamine HQ1-44, an iron chelator
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
McCoy’s 5a medium (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS (F2442).
Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3 x 10,0000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.

HEK 293T Cell Line

1. Catalog number: 12022001
2. Source: Human, fetal kidney cell
3. Cell type/morphology: Adherent, small slightly rounded cells growing in a dispersed manner
4. Application: Lentiviral particle production, expression of eukaryotic proteins, siRNA transfection, HIV transfection for pseudoviruses production
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
DMEM-Hi-glucose medium (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS (F2442).
Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e., seeding at 2-4 x 10,0000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were diluted 1:32 and counted using a Scepter™ 3.0 automated cell counter using 60 µm sensor.

HeLa Cell Line

1. Catalog number: 93021013
2. Source: Human cervix, epitheloid cervix carcinoma
3. Cell type/morphology: Adherent, epithelial
4. Application: To study Zika virus (ZIKV) infection; Virus studies; to study the cytotoxicity of extracts, fruits and jams from Sorbus sps
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
EMEM (EBSS) + 2 mM L-Glutamine (G7513) + 1% Non-Essential Amino Acids (NEAA)  + 10% FBS/FCS (F2442).
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3 x 10,0000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.

HT29 Cell Line

1. Catalog number: 91072201
2. Source: Human colon
3. Cell type/morphology: Adherent, epithelial
4. Application: HT29 Cell Line has been used to determine viral titers of human parechovirus. Study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717, Tumorigenicity studies
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B), and the 10X image with confluency mask (C).

8. Growth curve:

9. Cell culture media:
   McCoy′s5A (M8403) + 2 mM Glutamine (G7513) + 10% Fetal Bovine Serum FBS/FCS (F2442).
   Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.
10. Protocols: Cell culture protocols are available in the product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

Huh-7D12 Cell Line

1. Catalog number: 01042712
2. Source: Human liver
3. Cell type/morphology: Adherent, epithelial
4. Application: Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
DMEM-Hi-glucose medium (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS (F2442).
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 3-5 x 10,0000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were diluted 1:32 and counted using a Scepter™ 3.0 automated cell counter using 60 µm sensor.

IMR-90 Cell Line

1. Catalog number: 85020204
2. Source: Human lung (fetal)
3. Cell type/morphology: Adherent, fibroblast
4. Application: Virus studies, study the cytotoxicity of hydroxyapatite/gold /arginine (HAp/Au/arginine) nanoparticles.
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B), and the 10X image with confluency mask (C).

8. Growth curve:

9. Cell culture media:
   EMEM (EBSS) (M2279) + 2 mM Glutamine (TMS-002-C) + 1% Non-Essential Amino Acids (NEAA) (TMS-001-C) + 10% Fetal Bovine Serum (FBS) (ES-009-M).
   Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e. seeding at 2-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
   
10. Protocols: Cell culture protocols are available in the product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

LX-2 Cas9

1. Catalog number: SCC613
2. Source: Human liver
3. Cell type/morphology: Hepatic stellate cells, adherent
4. Application: Model of human hepatic fibrosis
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and 10X image with confluency mask (B)

8. Genetic profile: 

9. Cell culture media: Cells were cultured in DMEM (D5796) + 2% FBS (ES-009-B) + 1% L-Glutamine (TMS-002-C).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter using a 60 µm sensor.

MCF-7 Cell Line

1. Catalog number: 86012803
2. Source: Human, breast (adenocarcinoma)
3. Cell type/morphology: Adherent, epithelial-like
4. Application: Tumorigenicity and virus studies: type B and C viruses
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
EMEM (EBSS) + 2 mM L-Glutamine (G7513) + 1% Non-Essential Amino Acids (NEAA)  + 10% FBS/FCS (F2442).
Split sub-confluent cultures (70-80%) 1:2 to 1:6, i.e., seeding at 2-4 x 10,0000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.

MCF-7 BUS

1. Catalog number: SCC276
2. Source: Human breast cancer
3. Cell type/morphology: Adherent, epithelial
4. Application: Cellular model for estrogen-responsive metastatic breast cancer
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and 10X image with confluency mask (B)

8. Genetic profile:

9. Cell culture media: Cells were cultured in DMEM (D5796) + 10% FBS (ES-009-M).
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter using a 60 µm sensor.

MG-63 Cell Line

1. Catalog number: 86051601
2. Source: Human osteosarcoma
3. Cell type/morphology: Adherent, fibroblast
4. Application: Interferon-induction studies, to investigate the cytotoxicity of acrylic bone cement extracts on MG-63 cells, to fabricate three-dimensional (3-D) niches and study the cell viability, adhesion, and proliferation of MG-63 cells
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell® DCI at 10X (A), 20X (B), and the 10X image with confluency mask (C).

8. Growth curve:

9. Cell culture media:
   EMEM (EBSS) (M2279) + 2 mM Glutamine (TMS-002-C) + 1% Non-Essential Amino Acids (NEAA) (TMS-001-C) + 10% Fetal Bovine Serum (FBS) (ES-009-M).
   Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in the product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter.

MRC-5 SV2

1. Catalog number: 84100401
2. Source: Human, fetal lung cell
3. Cell type/morphology: Adherent, fibroblast-like fetal lung cell
4. Application: Transformation and virus studies: SV40
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
EMEM (EBSS) + 2 mM L-Glutamine (G7513) + 1% Non-Essential Amino Acids (NEAA)  + 10% FBS (F2442).
Split sub-confluent cultures (70-80%) 1:5 to 1:10, i.e., seeding at 1-2 x 10,0000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were diluted 1:8 and counted using a Scepter™ 3.0 automated cell counter using 60 µm sensor.

T24T Cell Line

1. Catalog number: SCC468
2. Source: Human bladder carcinoma
3. Cell type/morphology: Adherent, epithelial
4. Application: To study metastatic bladder cancer
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A) and 10X image with confluency mask (B)

8. Genetic profile:

9. Cell culture media: Cells were cultured in DMEM/F12 (D8062) + 10% FBS (ES-009-B)
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were counted on a Scepter™ 3.0 automated cell counter at 1:4 dilution.

WI38 Cell Line

1. Catalog number: 90020107
2. Source: Human, Female Caucasian, fetal lung cell
3. Cell type/morphology: Adherent, fibroblast-like fetal lung cell
4. Application: Virus studies (rhinoviruses), vaccine production, study cytotoxicity of acrylic based resin compounds and silica nanoparticles
5. Biosafety level: 2
6. Handling: Easy
7. Images: Brightfield image on Millicell^® DCI at 10X (A), 20X (B) and the 10X image with confluency mask (C).

8. Growth Curve

9. Cell culture media:
EMEM (EBSS) + 2 mM L-Glutamine (G7513) + 1% Non-Essential Amino Acids (NEAA)  + 15% FBS (F2442).
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4 x 10,0000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
Finite lifespan, 50 ± 10 population doublings.
10. Protocols: Cell culture protocols are available in product datasheet.
11. Scepter™ histogram: Cells were diluted 1:8 and counted using a Scepter™ 3.0 automated cell counter using 60 µm sensor.