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IMR-90 Cell Line human

85020204

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Synonym(s):
I90 Cells, IMR90 Cells, Institute for Medical Research-90 Cells
UNSPSC Code:
41106514

biological source

human lung (fetal)

description

Human Caucasian fetal lung fibroblast

growth mode

Adherent

karyotype

2n = 46, diploid

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian foetal lung fibroblast

Cell Line Description

Derived from lung tissue of a 16 week old female Caucasian foetus. The cells have a virus susceptibility similar to WI-38 (Sigma catalogue no. 90020107) and MRC-5 cells (Sigma catalogue no. 84101801). The cells have a finite lifespan. Available as a growing order only.

Application

IMR-90 human cell line has been used to study the cytotoxicity of hydroxyapatite/gold/arginine (HAp/Au/arginine) nanoparticles.
Virus studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,19

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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