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Huh-7D 12 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 01042712, human liver, Epithelial

Synonym(s):

Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells

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About This Item

UNSPSC Code:
41106514

biological source

human liver

packaging

tube of 5 μg 01042712-DNA-5UG
vial of  cells 01042712-1VL

growth mode

Adherent

karyotype

That of original Huh-7

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

storage temp.

−196°C

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This Item
CLS431144CLS430421CLS430183
Corning® Erlenmeyer cell culture flasks 125 mL Erlenmeyer Flask w/ Vent Cap, polycarbonate, sterile

CLS431143

Corning® Erlenmeyer cell culture flasks

Corning® Erlenmeyer cell culture flasks 250 mL Erlenmeyer Flask w/ Vent Cap, polycarbonate, sterile

CLS431144

Corning® Erlenmeyer cell culture flasks

Corning® Erlenmeyer cell culture flasks 125 mL Erlenmeyer Flask w/ Flat Cap, polycarbonate, sterile, 50/cs

CLS430421

Corning® Erlenmeyer cell culture flasks

Corning® Erlenmeyer cell culture flasks 250 mL Erlenmeyer Flask w/ Flat Cap, polycarbonate, sterile, 50/cs

CLS430183

Corning® Erlenmeyer cell culture flasks

manufacturer/tradename

Corning 431143

manufacturer/tradename

Corning 431144

manufacturer/tradename

Corning 430421

manufacturer/tradename

Corning 430183

material

clear polycarbonate flask, polypropylene cap (Easy-Grip vent), wide-mouth , flat bottom flask, wide-mouth neck, vent cap

material

clear polycarbonate flask, flat bottom flask, polypropylene cap (Easy-Grip vent), vent cap, wide-mouth , wide-mouth neck

material

clear polycarbonate flask, flat bottom flask, flat cap, polypropylene cap (Easy-Grip flat), wide-mouth , wide-mouth neck

material

clear polycarbonate flask, flat bottom flask, flat cap, polypropylene cap (Easy-Grip flat), wide-mouth , wide-mouth neck

feature

cap, graduations 25 mL, disposable, no baffles

feature

disposable, graduations 25 mL, with cap, no baffles

feature

cap, disposable, graduations 25 mL, no baffles

feature

cap, disposable, graduations 25 mL, no baffles

packaging

pack of 1

packaging

pack of 1, case of 50

packaging

pack of 1, case of 50

packaging

pack of 1, case of 50

flask capacity

125 mL

flask capacity

250 mL

flask capacity

125 mL

flask capacity

250 mL

General description

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.[1][2]

Cell Line Origin

Human hepatocellular carcinoma

Cell Line Description

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

Application

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

Culture Medium

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37 C. Media change twice a week

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

监管及禁止进口产品
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