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Safety Information

HT29 Cell Line human

91072201, human colon, Epithelial

Synonym(s):

HT29 Cells

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About This Item

UNSPSC Code:
41106514

Product Name

HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II

biological source

human colon

growth mode

Adherent

karyotype

2n = 46, hypertriploid

morphology

Epithelial

products

Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

Application

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.
Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

Culture Medium

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% Trypsin; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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