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Merck
CN

M8403

Sigma-Aldrich

McCoy 5A培养基

With sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

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别名:
5A basal medium
EC 号:
MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

运输

ambient

储存温度

2-8°C

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一般描述

McCoy 5A培养基适用于各种组织细胞原代培养。推荐用于在原代细胞培养中生产病毒。

应用

McCoy 5A培养基已用于维持:

  • G-361黑色素瘤细胞;
  • 87-MG细胞
  • 人结肠癌细胞系HCT116

生化/生理作用

McCoys 5A培养基起初是通过修改BME中的氨基酸,用来支持肝肿瘤细胞。 该配方还被用来支持骨髓、皮肤、牙龈、肾脏、大网膜、肾上腺、肺、脾、大鼠胚胎和其他细胞类型原代培养的生长。

重悬

补充 0.22 g/L L-谷氨酰胺。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Roman A Perez et al.
Journal of tissue engineering, 5, 2041731414543965-2041731414543965 (2014-11-11)
Developing appropriate cell culturing techniques to populate scaffolds has become a great challenge in tissue engineering. This work describes the use of spinner flask dynamic cell cultures to populate hydroxyapatite microcarriers for bone tissue engineering. The microcarriers were obtained through
Huw D Summers et al.
ACS nano, 7(7), 6129-6137 (2013-06-19)
Assessing dose in nanoparticle-cell interactions is inherently difficult due to a complex multiplicity of possible mechanisms and metrics controlling particle uptake. The fundamental unit of nanoparticle dose is the number of particles internalized per cell; we show that this can
Claudia De Fusco et al.
Journal of medicinal chemistry, 64(10), 6814-6826 (2021-04-27)
MAT2a is a methionine adenosyltransferase that synthesizes the essential metabolite S-adenosylmethionine (SAM) from methionine and ATP. Tumors bearing the co-deletion of p16 and MTAP genes have been shown to be sensitive to MAT2a inhibition, making it an attractive target for
A-P Sappino et al.
Oncogenesis, 1, e7-e7 (2012-01-01)
The p53 tumor-suppressor protein has a key role in the induction of cellular senescence, an important barrier to cancer development. However, very little is known about the physiological mediators of cellular senescence induced by p53. CEACAM1 is an immunoglobulin superfamily
Seungman Park et al.
Scientific reports, 9(1), 13286-13286 (2019-09-19)
We present a high-throughput microfluidics technique facilitating in situ measurements of cell mechanics parameters at the dorsal side of the cell, including molecular binding strengths, local traction forces, and viscoelastic properties. By adjusting the flow rate, the force magnitude exerted

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McCoy's 5A Modified Media Formulation

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