M8403
McCoy′s 5A Medium
With sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture
Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
5A basal medium
Recommended Products
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L
shipped in
ambient
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
McCoy′s 5A Medium is suitable for the primary culture of cells from a wide range of tissue. It is recommended for the production of viruses in primary cell cultures.
Application
McCoy′s 5A Medium has been used to maintain:
- G-361 melanoma cells;
- 87-MG cells
- human colon carcinoma cell line HCT116
Biochem/physiol Actions
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
Reconstitution
Supplement with 0.22 g/L L-glutamine.
also commonly purchased with this product
Product No.
Description
Pricing
related product
Product No.
Description
Pricing
supplement
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物来源培养基
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Roman A Perez et al.
Journal of tissue engineering, 5, 2041731414543965-2041731414543965 (2014-11-11)
Developing appropriate cell culturing techniques to populate scaffolds has become a great challenge in tissue engineering. This work describes the use of spinner flask dynamic cell cultures to populate hydroxyapatite microcarriers for bone tissue engineering. The microcarriers were obtained through
Huw D Summers et al.
ACS nano, 7(7), 6129-6137 (2013-06-19)
Assessing dose in nanoparticle-cell interactions is inherently difficult due to a complex multiplicity of possible mechanisms and metrics controlling particle uptake. The fundamental unit of nanoparticle dose is the number of particles internalized per cell; we show that this can
Claudia De Fusco et al.
Journal of medicinal chemistry, 64(10), 6814-6826 (2021-04-27)
MAT2a is a methionine adenosyltransferase that synthesizes the essential metabolite S-adenosylmethionine (SAM) from methionine and ATP. Tumors bearing the co-deletion of p16 and MTAP genes have been shown to be sensitive to MAT2a inhibition, making it an attractive target for
A-P Sappino et al.
Oncogenesis, 1, e7-e7 (2012-01-01)
The p53 tumor-suppressor protein has a key role in the induction of cellular senescence, an important barrier to cancer development. However, very little is known about the physiological mediators of cellular senescence induced by p53. CEACAM1 is an immunoglobulin superfamily
Seungman Park et al.
Scientific reports, 9(1), 13286-13286 (2019-09-19)
We present a high-throughput microfluidics technique facilitating in situ measurements of cell mechanics parameters at the dorsal side of the cell, including molecular binding strengths, local traction forces, and viscoelastic properties. By adjusting the flow rate, the force magnitude exerted
Articles
McCoy's 5A Modified Media Formulation
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service