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H9C2 (2-1) Cell Line from rat

BDIX heart myoblast, 88092904

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Synonym(s):
H9c2 Cells
UNSPSC Code:
41106514

biological source

rat cardiovascular

growth mode

Adherent

karyotype

Not specified

morphology

Myoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Rat BDIX heart myoblast

Cell Line Description

Skeletal muscle properties. Myotubes formed at confluency respond to acetylcholine. Differentiation improved by reducing serum concentration to 1%.

Application

Differentiation studies

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Important to passage at sub-confluence, reclone periodically to keep differentiated characteristics. Cells will fuse to form myotubes if allowed to reach confluency.

Other Notes

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Regulatory Information

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