Oli-neu Mouse Oligodendroglial Precursor Cell Line

Oli-neu is an immortalized mouse oligodendroglial cell line that recapitulates morphology and in vivo physiological activity of oligodendroglial cells. Oli-neu cells differentiate into oligodendrocytes upon daily addition of 1 mM dibutyrl-cAMP.2 Oli-neu cells have been utilized in a broad range of studies of oligodendrocyte biology and has greatly contributed to the understanding of the mechanisms of myelination and molecular players in myelination disorders. Differentiated Oli-neu oligodendrocytes express O4 antigen, an oligodendrocyte marker, and manifest cell processes characteristic of oligodendrocyte morphology.2 The Oli-neu cell line is a versatile cellular model for oligodendrocyte biology and associated neurophysiological investigations.

Oligodendrocytes, comprising 5-10% of the total population of glial cells in the central nervous system, are responsible for producing the myelin sheath encasing axons which is essential for efficient electric conduction of neural impulses.1 Oligodendrocytes arise from precursor cells that differentiate just before birth. Disorders of myelination of axons can lead to emergence of neurodegenerative diseases such as Alzheimer′s and multiple sclerosis, underscoring the critical importance of understanding oligodendrocyte biology and interactions. The myelination process occurs within the complex milieu of neuronal cell types, thus is difficult to reconstruct without cellular models. Oli-neu is an immortalized mouse oligodendroglial cell line that recapitulates morphology and in vivo physiological activity of oligodendroglial cells. Oli-neu cells differentiate into oligodendrocytes upon daily addition of 1 mM dibutyrl-cAMP.2 Oli-neu cells have been utilized in a broad range of studies of oligodendrocyte biology and has greatly contributed to the understanding of the mechanisms of myelination and molecular players in myelination disorders. Differentiated Oli-neu oligodendrocytes express O4 antigen, an oligodendrocyte marker, and manifest cell processes characteristic of oligodendrocyte morphology.2 The Oli-neu cell line is a versatile cellular model for oligodendrocyte biology and associated neurophysiological investigations.Source Oli-neu was derived from oligodendrocytes and oligodendrocyte precursor cells isolated from the cerebellum of a 6-day-old NMRI mouse and infected with retrovirus containing the t-neu tyrosine kinase oncogene.2References1. Curr Pharm Des. 2016, 22(6): 656-679.2. Eur J Neurosci. 1995, 7(6): 1245-1265.

The Oli-neu cell line is a versatile cellular model for oligodendrocyte biology and associated neurophysiological investigations.

Oli-neu mouse oligodendrocyte precursor cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting functionality.

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