Mouse OP9-DL4 Delta-like 4 Notch Ligand Cell Line

OP9-DL4 is a stable bone marrow derived stromal cell line expressing the Notch ligand- Delta like 4 (DL4) ectopically.Notch signaling controls multiple cell-fate decisions. Four Notch receptors (Notch 1- Notch 4) have been identified in mammals. These receptors interact with Jagged or Delta-like (DL) family members of Notch ligands. This is followed by cleavage of the intracellular domain of Notch and its subsequent translocation into the nucleus, where it binds with transcription factors and activates transcription of various downstream target genes. Notch signaling is essential in early T- cell lineage commitments. Bone marrow progenitor cells expressing constitutively-active Notch develop into CD4 and CD8 double positive T cells rather than B cells. They also play an important role in development of CD4+ and CD8+ single positive cells from double positive precursor thymocytes, in addition to development of T-cells with αβ+ TCRs. Of the 4 Notch receptors, Notch1 (N1) receptor signaling has been shown to be sufficient for T cell development. The delta like ligands are physiologically relevant N1 ligands. DL1 interacts with both N1 and Notch 2 (N2) to induce T- cell lineage commitment. However, DL4 interacts specifically only with N1 and supports T cell commitment and maturation both in vitro and in vivo. Moreover, results from previous binding studies show that binding between DL1 and N1 is weak as opposed to the stronger binding between DL4 and N1. Of the DL1 and DL4 delta like ligands, DL4 exhibits greater capacity to activate the Notch pathway in hematopoietic progenitor cells. SourceOP9-DL4 cells were genetically modified from OP9 stromal cells derived from mouse bone marrow.References1.Immunity 2002, 17(6): 749-756.2.J Orthop Sci. 2005, 10(6): 589-594.3.Curr Opin Immunol. 2007, 19(2): 163-168. 4.Cold Spring Harb Protoc. 2009, 2009(2): pdb.prot5156.

Mouse OP9-DL4 cells express the Notch ligand Delta-like 4 and have been demonstrated to promote T cell lineage commitment in hematopoietic stem cells. These cells support the differentiation of embryonic or hematopoietic stem cells from fetal liver or bone marrow into T lymphocytes. The OP9-DL4 cell line is an essential tool for elucidating Notch signaling in cellular differentiation and the initiation of inflammatory disease states.

OP9-DL4 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.

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