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Protein Molecular Weight Calibration Curve on Zenix® Size Exclusion Columns, Effect of Pore Size

Protein Molecular Weight Calibration Curve on Zenix® Size Exclusion Columns, Effect of Pore Size application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Zenix® SEC-150 Gel Filtration Column, 3 μm

L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steel

Zenix® SEC-300 Gel Filtration Column, 3 μm

L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steel

standard

Product No.
Description
Pricing

γ-Globulins from human blood

≥99% (electrophoresis)

Myoglobin from equine heart

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

Vitamin B12

≥98%

Thyroglobulin from bovine thyroid

powder, ≥90% (agarose gel electrophoresis)

Bovine Serum Albumin

lyophilized powder, ≥96% (agarose gel electrophoresis)

Ribonuclease A from bovine pancreas

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

Ovalbumin (257-264) chicken

≥97% (HPLC)

CONDITIONS

column

Zenix SEC-150, 30 cm x 7.8 mm I.D., 3 μm, 150 Å particles (Z777018)

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm, 300 Å particles (Z777033)

mobile phase

150 mM sodium phosphate, pH 7

flow rate

1 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

10 μL

sample

each 1 mg/mL except uracil, 2.5 mg/mL

Description

Legal Information

Zenix is a registered trademark of Sepax Technologies