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HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)

HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1) application for HPLC

Materials

standard

Product No.
Description
Pricing

T1021

Trypsin inhibitor from Glycine max (soybean)

Isoelectric focusing marker, pI 4.6

T9767

Trypsin inhibitor from Glycine max (soybean)

BioReagent, For use as a marker in SDS-PAGE

T9003

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder

T9128

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder

T6522

Trypsin inhibitor from Glycine max (soybean)

powder, BioReagent, suitable for cell culture

M1882

Myoglobin from equine heart

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

M9267

Myoglobin from equine heart

Isoelectric focusing marker, pI (1) 6.8, (2) 7.2

M6036

Myoglobin from human heart

≥95% (SDS-PAGE), buffered aqueous glycerol solution

T0256

Trypsin inhibitor from bovine pancreas

Type I-P, essentially salt-free, lyophilized powder

C0880

Conalbumin from chicken egg white

Iron complex

C7786

Conalbumin from chicken egg white

BioReagent, suitable for cell culture

C0755

Conalbumin from chicken egg white

Substantially iron-free

T9378

Trypsin inhibitor from Phaseolus limensis (lima bean)

Type II-L, crude powder

T6414

Trypsin inhibitor from Glycine max (soybean)

solution, sterile-filtered, suitable for, suitable for cell culture

M0630

Myoglobin from equine skeletal muscle

95-100%, essentially salt-free, lyophilized powder

T2011

Trypsin inhibitor from chicken egg white

Type III-O (free of ovoinhibitor)

T9253

Trypsin inhibitor from chicken egg white

Type II-O, Partially purified ovomucoid, containing ovoinhibitor

93620

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder, ~10000 U/mg

M5696

Myoglobin from equine skeletal muscle

BioUltra, 95-100% (SDS-PAGE)

T2327

Trypsin Inhibitor from Glycine max (soybean)

BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

CONDITIONS

column

Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)

mobile phase

[A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)

flow rate

0.5 mL/min

detector

UV, 280 nm

injection

10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A

Description

Analysis Note

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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