Skip to Content

很抱歉,目前系统维护中。在此期间,网站下单功能无法使用。给您带来不便,深表歉意。如需其他帮助,请联系客户联络中心:400 620 3333。

Merck
CN
  • Structural insights into the assembly of the histone deacetylase-associated Sin3L/Rpd3L corepressor complex.

Structural insights into the assembly of the histone deacetylase-associated Sin3L/Rpd3L corepressor complex.

Proceedings of the National Academy of Sciences of the United States of America (2015-07-01)
Michael D Clark, Ryan Marcum, Richard Graveline, Clarence W Chan, Tao Xie, Zhonglei Chen, Yujia Ding, Yongbo Zhang, Alfonso Mondragón, Gregory David, Ishwar Radhakrishnan
ABSTRACT

Acetylation is correlated with chromatin decondensation and transcriptional activation, but its regulation by histone deacetylase (HDAC)-bearing corepressor complexes is poorly understood. Here, we describe the mechanism of assembly of the mammalian Sin3L/Rpd3L complex facilitated by Sds3, a conserved subunit deemed critical for proper assembly. Sds3 engages a globular, helical region of the HDAC interaction domain (HID) of the scaffolding protein Sin3A through a bipartite motif comprising a helix and an adjacent extended segment. Sds3 dimerizes through not only one of the predicted coiled-coil motifs but also, the segment preceding it, forming an ∼ 150-Å-long antiparallel dimer. Contrary to previous findings in yeast, Sin3A rather than Sds3 functions in recruiting HDAC1 into the complex by engaging the latter through a highly conserved segment adjacent to the helical HID subdomain. In the resulting model for the ternary complex, the two copies of the HDACs are situated distally and dynamically because of a natively unstructured linker connecting the dimerization domain and the Sin3A interaction domain of Sds3; these features contrast with the static organization described previously for the NuRD (nucleosome remodeling and deacetylase) complex. The Sds3 linker features several conserved basic residues that could potentially maintain the complex on chromatin by nonspecific interactions with DNA after initial recruitment by sequence-specific DNA-binding repressors.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Imidazole, ReagentPlus®, 99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
¥241.79
25 g
Available to ship on 2025年4月07日
Details...
¥564.48
100 g
Available to ship on 2025年4月07日
Details...
¥1,047.58
500 g
Available to ship on 2025年4月07日
Details...
¥1,986.60
1 kg
Available to ship on 2025年4月07日
Details...
¥3,854.00
2 kg
Available to ship on 2025年4月07日
Details...
¥6,012.89
5 kg
Available to ship on 2025年4月07日
Details...
¥14,455.73
Sigma-Aldrich
Imidazole, puriss. p.a., ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 g
Available to ship on 2025年4月07日
Details...
¥960.92
500 g
Available to ship on 2025年4月07日
Details...
¥1,648.78
¥1,154.15
1 kg
Available to ship on 2025年4月07日
Details...
¥3,141.77
Sigma-Aldrich
Magnesium chloride solution, BioUltra, for molecular biology, 2 M in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Available to ship on 2025年4月07日
Details...
¥1,253.64
¥803.11
Sigma-Aldrich
Magnesium chloride solution, BioUltra, for molecular biology, ~1 M in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Available to ship on 2025年4月07日
Details...
¥1,840.19
¥1,178.88
500 mL
Available to ship on 2025年4月07日
Details...
¥6,823.96
Sigma-Aldrich
Imidazole, BioUltra, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 g
Available to ship on 2025年4月07日
Details...
¥5,167.63
250 g
Please contact Customer Service for Availability
¥14,445.51
1 kg
Please contact Customer Service for Availability
¥33,667.36
Sigma-Aldrich
Imidazole, BioUltra, for molecular biology, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 g
Please contact Customer Service for Availability
¥5,594.43
250 g
Please contact Customer Service for Availability
¥18,851.13
Sigma-Aldrich
Imidazole, for molecular biology, ≥99% (titration)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Available to ship on 2025年4月07日
Details...
¥659.04
25 g
Available to ship on 2025年4月07日
Details...
¥3,460.70
100 g
Available to ship on 2025年4月07日
Details...
¥10,363.42
Sigma-Aldrich
Monoclonal Anti-c-Myc antibody produced in mouse, clone 9E10, ascites fluid
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 μL
Please contact Customer Service for Availability
¥4,703.53
0.2 mL
Available to ship on 2025年4月07日
Details...
¥6,171.31
Sigma-Aldrich
Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 vial
Available to ship on 2025年4月07日
Details...
New, lower price on this item!
¥285.72
5 mL
Available to ship on 2025年4月07日
Details...
New, lower price on this item!
¥727.84
5 vials
Available to ship on 2025年4月07日
Details...
¥971.57
Sigma-Aldrich
Imidazole, ≥99% (titration), crystalline
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Available to ship on 2025年4月07日
Details...
¥540.64
25 g
Please contact Customer Service for Availability
¥2,398.66
100 g
Available to ship on 2025年4月07日
Details...
¥5,535.09
250 g
Please contact Customer Service for Availability
¥11,842.46
Sigma-Aldrich
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
0.2 mg
Available to ship on 2025年4月07日
Details...
¥7,230.05
1 mg
Available to ship on 2025年4月07日
Details...
¥13,937.30
5 mg
Available to ship on 2025年4月07日
Details...
¥36,660.77
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
240 mL
Estimated to ship on 2025年6月12日
Details...
¥969.85
Sigma-Aldrich
Imidazole, ACS reagent, ≥99% (titration)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 g
Available to ship on 2025年4月07日
Details...
¥835.60
500 g
Please contact Customer Service for Availability
¥2,593.00
Sigma-Aldrich
Anti-HA antibody, Mouse monoclonal, clone HA-7, purified from hybridoma cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 μL
Available to ship on 2025年4月07日
Details...
¥6,023.96
200 μL
Available to ship on 2025年4月07日
Details...
¥7,286.29
Sigma-Aldrich
Magnesium chloride solution, 0.1 M
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 mL
Please contact Customer Service for Availability
¥720.61
Sigma-Aldrich
Imidazole, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 g
Available to ship on 2025年4月07日
Details...
¥638.27
500 g
Available to ship on 2025年4月07日
Details...
¥2,668.08
1 kg
Available to ship on 2025年4月07日
Details...
¥3,525.15
Sigma-Aldrich
Imidazole, for molecular biology, ≥99% (titration), free-flowing, Redi-Dri
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 g
Estimated to ship on 2025年5月06日
Details...
¥6,272.89
Sigma-Aldrich
Imidazole, ReagentPlus®, 99%, Redi-Dri, free-flowing
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 g
Estimated to ship on 2025年6月03日
Details...
¥544.66
500 g
Please contact Customer Service for Availability
¥1,844.87
1 kg
Estimated to ship on 2025年5月23日
Details...
¥3,158.62
2.5 kg
Please contact Customer Service for Availability
¥5,414.76
Sigma-Aldrich
Imidazole, Vetec, reagent grade, 98%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
Available to ship on 2025年4月07日
Details...
¥371.83
Sigma-Aldrich
Magnesium chloride solution, BioUltra, for molecular biology, ~0.025 M in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
10 x 1 mL
Please contact Customer Service for Availability
¥680.12
Sigma-Aldrich
Magnesium chloride solution, for molecular biology, 1.00 M±0.01 M
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
10 x 1 mL
Available to ship on 2025年4月07日
Details...
¥918.20
100 mL
Available to ship on 2025年4月07日
Details...
¥1,094.56
Sigma-Aldrich
Ethanol, absolute, sales not in Germany, ≥99.8% (vol.)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 L
Please contact Customer Service for Availability
¥1,501.14
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Available to ship on 2025年4月07日
Details...
¥294.44
500 mL
Available to ship on 2025年4月07日
Details...
¥927.35
1 L
Available to ship on 2025年4月07日
Details...
¥1,240.23
2 L
Available to ship on 2025年4月07日
Details...
¥1,995.05
6 x 1 L
Please contact Customer Service for Availability
¥6,657.52
Sigma-Aldrich
Magnesium chloride, AnhydroBeads, −10 mesh, 99.9% trace metals basis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
10 g
Available to ship on 2025年4月07日
Details...
¥1,255.81
50 g
Please contact Customer Service for Availability
¥3,037.49
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 L
Available to ship on 2025年4月07日
Details...
¥1,181.02
Sigma-Aldrich
Magnesium chloride, powder, <200 μm
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 g
Available to ship on 2025年4月07日
Details...
¥338.33
1 kg
Available to ship on 2025年4月07日
Details...
¥568.18
¥340.91
5 kg
Please contact Customer Service for Availability
¥2,063.23
Sigma-Aldrich
Magnesium chloride, AnhydroBeads, −10 mesh, 99.99% trace metals basis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
10 g
Available to ship on 2025年4月07日
Details...
¥1,422.07
50 g
Available to ship on 2025年4月07日
Details...
¥5,239.28
Sigma-Aldrich
Imidazole buffer Solution, BioUltra, 1 M in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Available to ship on 2025年4月07日
Details...
¥1,237.75
500 mL
Please contact Customer Service for Availability
¥3,275.60
Sigma-Aldrich
Magnesium chloride, anhydrous, ≥98%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 g
Available to ship on 2025年4月07日
Details...
¥1,004.34
¥703.04
1 kg
Available to ship on 2025年4月07日
Details...
¥4,912.40
¥3,384.53
Supelco
Ethanol standards 10% (v/v), 10 % (v/v) in H2O, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
10 amp
Please contact Customer Service for Availability
¥1,616.01