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M8787

Sigma-Aldrich

Magnesium chloride solution

PCR Reagent, 25 mM MgCI2 solution for PCR

Synonym(s):

PCR optimization

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About This Item

Linear Formula:
MgCl2
CAS Number:
7786-30-3
Molecular Weight:
95.21
MDL number:
MFCD00011106
UNSPSC Code:
12352302
PubChem Substance ID:
57654422
NACRES:
NA.52

grade

PCR Reagent

Quality Level

200
300

form

liquid

packaging

vial of 1.5 mL

concentration

25 mM±1 mM

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture
diagnostic assay manufacturing

foreign activity

DNase, RNase, none detected

storage temp.

2-8°C

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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General description

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Application

Magnesium chloride solution has been used as a component of the:

  • amplification mixture for polymerase chain reaction (PCR)
  • amplification mixture in an inter simple sequence repeats (ISSR)-PCR
  • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
  • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
  • reaction mix for RT-PCR to assess the gene expression

Suitability

Suitable for optimization of polymerase chain reactions.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Molecular characterization of Fusarium oxysporum f. sp. cubense isolates from banana
Das A, et al.
Pest Management in Horticultural Ecosystems , 18(2), 171-178 (2012)
Massimiliano Bergallo et al.
American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Genome subtyping of autochthonous Bacillus species isolated from Iru, a fermented Parkia biglobosa seed
Adewumi G A, et al.
Food Biotechnology, 28(3), 250-268 (2014)
Fernando Cartón-García et al.
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches
Benjamin Dombert et al.
PloS one, 9(10), e110846-e110846 (2014-10-23)
Spinal muscular atrophy (SMA) is caused by deficiency of the ubiquitously expressed survival motoneuron (SMN) protein. SMN is crucial component of a complex for the assembly of spliceosomal small nuclear ribonucleoprotein (snRNP) particles. Other cellular functions of SMN are less
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