M5546
Monoclonal Anti-c-Myc antibody produced in mouse
clone 9E10, ascites fluid
Synonym(s):
Anti-c-Myc
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
9E10, monoclonal
contains
15 mM sodium azide
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:100 using c-myc tagged fusion protein
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MYC(4609)
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General description
MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. c-myc is a member of MYC gene family. It is located on human chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor.
Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.
Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.
c-Myc is a transcription factor which is over-expressed in a wide range of cancers.
Specificity
Recognizes both N- terminal and C-terminal c-Myc tagged proteins.
Immunogen
synthetic peptide of the human p62c-Myc protein.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Monoclonal anti-c-Myc antibody produced in mouse has been used for Western blotting and immunohistochemistry.
Monoclonal Anti-c-Myc antibody has also been used in:
Monoclonal Anti-c-Myc antibody has also been used in:
- the detection of horseradish peroxidase conjugate in phosphate buffered saline tween-20 (PBST) and 65-kDa mannoprotein (MP65) Dabs
- immunoblotting
- immunoprecipitation
- biochemical and histological studies
Biochem/physiol Actions
The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as a transcriptional regulator of gene expression. MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in the poor outcome and invasive treatment, when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP).
c-Myc is a regulator of the cell cycle and aids in the growth of tumors in the liver. Translocations in the gene encoding it have been shown to be linked to Burkitt lymphoma. It has been studied that c-Myc targets many protein-coding genes which enhances the oncogenic properties of c-Myc.
Physical form
The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C for a maximum of one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Regulatory Information
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Certificates of Analysis (COA)
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A PTK7/Ror2 Co-receptor complex affects xenopus neural crest migration.
Podleschny M, et al.
PLoS ONE, 10(12), e0145169-e0145169 (2015)
Structure-function analysis of Nel, a thrombospondin-1-like glycoprotein involved in neural development and functions.
Nakamura R, et al.
The Journal of Biological Chemistry, 287(5), 3282-3291 (2012)
Clinicopathological significance of c-MYC in esophageal squamous cell carcinoma.
Lian Y, et al.
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 39(7), 1010428317715804-1010428317715804 (2017)
MYC Protein?positive Diffuse Large B-Cell Lymphoma Features an Activated B-Cell Receptor Signal Pathway.
Wang WG, et al.
American Journal of Surgical Pathology, 41(4), 541-549 (2017)
8q24 prostate, breast, and colon cancer risk loci show tissue-specific long-range interaction with MYC.
Ahmadiyeh N, et al.
Proceedings of the National Academy of Sciences of the USA, 107(21), 9742-9746 (2010)
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