Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Toxicology mechanisms and methods (2015-01-07)

E Brindha, M Rajasekapandiyan

ABSTRACT

This study was aimed to evaluate the preventive role of phytic acid on lysosomal enzymes in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats subcutaneously injected with ISO (85 mg/kg) at an interval of 24 h for two days showed a significant increase in the activities of lysosomal enzymes (glucuronidase, N-acetyl glucosaminidase, galactosidase, cathepsin-B and cathepsin-D) were increased significantly in serum and the heart of ISO-induced rats, but the activities of glucuronidase and cathepsin-D were decreased significantly in the lysosomal fraction of the heart. Pretreatment with phytic acid (25 and 50 mg/kg) daily for a period of 56 d positively altered activities of lysosomal hydrolases in ISO-induced rats. Thus, phytic acid possesses a cardioprotective effect in ISO-induced MI in rats.

MATERIALS

Product Number

Brand

Product Description

L4509

Sigma-Aldrich

Sodium dodecyl sulfate, ReagentPlus^®, ≥98.5% (GC)

L5750

Sigma-Aldrich

Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

M1028

Sigma-Aldrich

Magnesium chloride solution, for molecular biology, 1.00 M±0.01 M

H9892

Sigma-Aldrich

Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture

M8787

Sigma-Aldrich

Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂solution for PCR

L6026

Sigma-Aldrich

Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

71726

Supelco

Sodium dodecyl sulfate, suitable for ion pair chromatography, LiChropur^™, ≥99.0%

71725

Sigma-Aldrich

Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)

71717

Sigma-Aldrich

Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

62862

Sigma-Aldrich

Sodium dodecyl sulfate, ≥90% ((Assay))

63069

Sigma-Aldrich

Magnesium chloride solution, BioUltra, for molecular biology, ~1 M in H₂O

17934

Supelco

Hydrogen chloride – ethanol solution, ~1.25 M HCl, for GC derivatization, LiChropur^™

17935

Supelco

Hydrogen chloride – methanol solution, ~1.25 m HCl (T), for GC derivatization, LiChropur^™

71383

Sigma-Aldrich

Sodium chloride, tested according to Ph. Eur.

17933

Supelco

Hydrogen chloride – 2-propanol solution, ~1.25 M HCl (T), for GC derivatization, LiChropur^™

84429

Sigma-Aldrich

Hydrochloric acid solution, ~6 M in H₂O, for amino acid analysis

71387

Supelco

Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%

436143

Sigma-Aldrich

Sodium dodecyl sulfate, ACS reagent, ≥99.0%

63036

Sigma-Aldrich

Magnesium chloride solution, BioUltra, for molecular biology, ~0.025 M in H₂O

71729

Sigma-Aldrich

Sodium dodecyl sulfate, ≥98.0% (GC)

PHR1321

Supelco

Sodium chloride, Pharmaceutical Secondary Standard; Certified Reference Material

75746

Sigma-Aldrich

Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets

74255

Supelco

Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)

00457

Sigma-Aldrich

Magnesium chloride solution, 0.1 M

V900859

Sigma-Aldrich

Sodium dodecyl sulfate, Vetec^™, reagent grade, ≥98%

440469

Sigma-Aldrich

Lithium lactate, 95%

RDD040

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC), free-flowing, Redi-Dri^™

339253

Sigma-Aldrich

Hydrochloric acid, 37 wt. % in H₂O, 99.999% trace metals basis

258148

Sigma-Aldrich

Hydrochloric acid, ACS reagent, 37%

378860

Sigma-Aldrich

Sodium chloride, random crystals, optical grade, 99.9% trace metals basis