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L3771

Sigma-Aldrich

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

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Synonym(s):
Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
151-21-3
Molecular Weight:
288.38
Beilstein:
3599286
EC Number:
205-788-1
MDL number:
MFCD00036175
UNSPSC Code:
12161902
eCl@ss:
39093306
PubChem Substance ID:
24896351
NACRES:
NB.22

biological source

synthetic

Quality Level

200

grade

for molecular biology

description

anionic

product line

BioReagent

Assay

≥98.5% (GC)

form

powder

mol wt

micellar 18,000

aggregation number

62

technique(s)

electrophoresis: suitable

impurities

≤2.0% water (Karl Fischer)

CMC

7-10 mM (20-25°C)

mp

204-207 °C (lit.)

transition temp

cloud point >100 °C

solubility

water: soluble

density

1.03 g/cm3 at 20 °C (68 °F)

anion traces

chloride (Cl-): ≤500 ppm
phosphate (PO43-): ≤10 ppm

cation traces

heavy metals (as Pb): ≤10 ppm

absorption

≤0.1 at 260 at 3%
≤0.1 at 280 at 3%

HLB

40

suitability

suitable for electrophoresis

foreign activity

DNase, RNase, none detected

storage temp.

room temp

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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General description

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is an anionic surfactant widely used in various scientific applications, particularly in molecular biology, biochemical, and cell biology research. Its effectiveness stems from its amphiphilic nature, allowing it to interact with both hydrophobic and hydrophilic molecules. SDS is a versatile detergent that excels in solubilizing and denaturing proteins, making it an essential tool in protein extraction and purification procedures. By disrupting protein structures, SDS facilitates the separation of proteins based on their molecular weight during SDS-PAGE electrophoresis.

Beyond protein analysis, SDS also finds applications in the electrophoretic separation of lipids, enabling the study and characterization of these important cellular components. Additionally, SDS is employed in various hybridization and molecular biology techniques, demonstrating its versatility across a range of research areas. SDS′s effectiveness in both ionic and anionic conditions further expands its utility, allowing it to adapt to diverse experimental setups and requirements. Its ability to unravel proteins by binding to peptide chains and imparting a negative charge effectively eliminates protein shape as a factor for gel separation, ensuring accurate protein size determination.

Application

Sodium dodecyl sulfate has been used:
  • in chromatin immunoprecipitation
  • in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
  • as a component of radioimmunoprecipitation assay buffer for immunoblotting
Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.
Anionic detergent

Features and Benefits

  • Highly versatile surfactant for your molecular biology and biochemical research
  • Suitable for electrophoresis applications
  • Tested to confirm low levels of heavy metal contamination,ensuring suitability for various applications.

Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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comparable product

Product No.
Description
Pricing

L5750

Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

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Sodium dodecyl sulfate, ReagentPlus®, ≥98.5% (GC)

L6026

Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

71717

Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

62862

Sodium dodecyl sulfate, ≥90% ((Assay))

71725

Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)

71729

Sodium dodecyl sulfate, ≥98.0% (GC)

71736

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H2O

55422

Sodium dodecyl sulfate, ≥98.0% (GC)

05030

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 20% in H2O

436143

Sodium dodecyl sulfate, ACS reagent, ≥99.0%

75746

Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets

RDD021

Sodium dodecyl sulfate, anhydrous, free-flowing, Redi-Dri™, ACS reagent, ≥99.0%

RDD040

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC), free-flowing, Redi-Dri™

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 2

Flash Point(F)

338.0 °F

Flash Point(C)

170 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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