G005689
LC/MS Analysis of Conjugated Bile Acids on Ascentis® Express RP-Amide
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express RP-Amide, 5 cm x 4.6 mm I.D., 2.7 μm particles (53922-U)
mobile phase: [A] 10 mM ammonium acetate, pH 3 with acetic acid; [B] acetonitrile
gradient: 25 to 40% B in 10 min
flow rate: 1.0 mL/min (postcolumn split to the MS)
column temp.: 35 °C
detector: MS, ESI(-), SIR
injection: 5 μL
sample: 10 mg/L in mobile phase A
suitability
application for HPLC
application(s)
clinical
1 of 4
This Item | MABN863 | AB5158 | AB5152 |
|---|---|---|---|
| biological source rabbit | biological source mouse | biological source rabbit | biological source rabbit |
| antibody form affinity purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity purified immunoglobulin | antibody form affinity purified immunoglobulin |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| clone polyclonal | clone 8F9.1, monoclonal | clone polyclonal | clone polyclonal |
| technique(s) immunohistochemistry: suitable, western blot: suitable, immunoprecipitation (IP): suitable | technique(s) immunohistochemistry: suitable (paraffin), western blot: suitable | technique(s) immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable |
Legal Information
analytical column
mobile phase component
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